Application of soybean peptide in preparing medicine for controlling airway inflammatory reaction
A technology of airway inflammation and soybean peptide, which is applied in the field of soybean peptide in the preparation of drugs for the prevention and treatment of airway inflammation. It can solve the problems that soybean peptide has not been reported, and achieve the effect of improving pathological damage and reducing airway inflammation
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[0015] The present invention will be further described below in conjunction with some specific examples.
[0016] 1 Materials and methods
[0017] 1.1 Experimental animals
[0018] SD rats (SPF grade), male, 8 weeks old, weighing 180-200g, were adaptively fed in the animal room for 5 days, 4-5 per cage, and the litter was changed every 3-4 days, and the indoor temperature was adjusted to 22 ± 2°C, humidity 45%-65%, 12h light and dark photoperiod, regular feeding, free drinking water.
[0019] 1.2 Main reagents
[0020] Soybean peptide, pentobarbital sodium ovalbumin, aluminum hydroxide, rabbit anti-Notch 1, Jagged 1, Hes 1, NF-κB and β-actin antibodies, goat anti-rabbit horseradish peroxidase (horseradish peroxidase, HRP ) labeled secondary antibody, enhanced RIPA protein lysate, BCA protein concentration determination kit, color pre-stained protein molecular weight standard (10-180KDa), PVDF membrane and SDS-PAGE gel preparation kit, Trizol total RNA extraction reagent, E...
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