Lactococcus lactis subsp. lactis CCFM1018, fermented food thereof and application thereof to medicament preparation

A technology of Lactococcus lactis and lactic acid subspecies, applied in the field of microorganisms, can solve the problems of unsatisfactory adsorption effect, achieve good gastric acid resistance and bile salt resistance, reduce content, and reduce damage effects

Active Publication Date: 2018-09-25
无锡食生臻选生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing literature reports, the highest adsorption rate of probiotics to DEHP in vitro is only 9.62%, and the adsorption effect is not ideal.

Method used

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  • Lactococcus lactis subsp. lactis CCFM1018, fermented food thereof and application thereof to medicament preparation
  • Lactococcus lactis subsp. lactis CCFM1018, fermented food thereof and application thereof to medicament preparation
  • Lactococcus lactis subsp. lactis CCFM1018, fermented food thereof and application thereof to medicament preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Lactococcus lactis subsp. lactis CCFM1018 has good tolerance to simulated gastrointestinal fluid

[0069] The cryopreserved Lactococcus lactis subsp. lactis CCFM1018 was streaked in a solid medium, incubated aerobic at a temperature of 30°C for 24 hours, and then subcultured in liquid medium for 2 to 3 times. Culture medium of CCFM1018, centrifuged at 8000×g for 5 min to collect the bacteria, resuspended in (1:1) pH2.5 artificial simulated gastric juice (containing 1% pepsin, pH=2.5 MRS medium) and mixed, and then at 37℃ Under aerobic culture, samples were taken at the beginning (0h), 1h, 2h, and 3h respectively, and the plate colonies were counted by pouring and culturing with MRS agar medium to determine the number of viable bacteria and calculate their survival rate. The survival rate is the ratio of the number of viable cells in the culture solution to the number of viable cells at 0 h, expressed in %.

[0070] Take the culture broth of Lactococcus lactis sub...

Embodiment 2

[0075] Example 2: Lactococcus lactis subsp. lactic acid CCFM1018 has good adsorption capacity for DEHP and MEHP in an aqueous solution containing plasticizer DEHP or MEHP in vitro

[0076] Bacterial adsorption: After purifying and cultivating experimental bacteria, they are connected to MRS liquid medium according to 1% (v / v) inoculum, and cultured at 30°C for 20h (E.coli is cultured with LB medium at 37°C with shaking; E. .faecalis is cultured in MRS liquid medium at 37℃), then centrifuged at 8000×g for 20 min, discard the supernatant, resuspend in ultrapure water, and continue to centrifuge at 8000×g for 20 min, discard the supernatant. Live bacteria cells, that is, wet bacteria. Suspend the wet bacteria body weight in 50mg / LDEHP or 10mg / L MEHP water solution, and make the final bacterial cell concentration reach 1g wet bacteria body / L. The blank control is the wet bacteria body weight suspension in ultrapure water without DEHP and MEHP. The volume of each group is 1 mL. The ...

Embodiment 3

[0083] Example 3: Lactococcus lactis subsp. lactis CCFM1018 has no acute side effects on SD rats

[0084] Resuspend Lactococcus lactis subsp. lactis CCFM1018 in a 2% (w / v) sucrose solution with a cell density of 1.0×10 9 CFU / mL. Take 10 healthy male SD rats with a weight of about 100g, give 2 mL of this concentration suspension daily, observe for a week, and record the death and body weight.

[0085] The results of these tests are listed in Table 4. The results show that the feeding concentration is 1.0×10 9 The CFU / mL Lactococcus lactis subsp. lactis CCFM1018 did not cause significant effects on the rats, the body weight did not change significantly, and there was no death. The rat has no obvious pathological symptoms.

[0086] Table 4 Changes in body weight and death of rats

[0087]

[0088] Note: -: No rats died

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Abstract

The invention discloses lactococcus lactis subsp. lactis CCFM1018, a fermented food thereof and application thereof to medicament preparation. The lactococcus lactis subsp. lactis not only is obviously superior to intestinal resident flora such as escherichia coli and enterococcus faecalis in the aspect of an effect of promoting discharge of DEHP (Di 2-Ethyl Hexyl Phthalate) and MEHP (mono-2-EthylHexyl Phthalate), but also is superior to a commercial strain of LGG (Lactobacilus rhamnosus GG). Therefore, the lactococcus lactis subsp. lactis CCFM1018 disclosed by the invention can be used as aneffective means for preventing and relieving body damage caused by DEHP and MEHP, and has no toxic or side effects of medicines. The lactococcus lactis subsp. lactis CCFM1018 can be used for preparing a medicinal composition and the fermented food which are used for relieving and preventing toxicity of DEHP and metabolites thereof, and has very wide application prospect.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to Lactococcus lactis subsp. lactis CCFM1018, its fermented food and its application in the preparation of medicines. Background technique [0002] As a plasticizer, bis(2-ethylhexyl) phthalate (DEHP) can be added to plastics to increase its flexibility and plasticity. Due to its low price, DEHP is widely used in the production of plastic products such as medical equipment and chemical products. It is currently the most widely used plasticizer in my country. In these plastic products, DEHP is mainly combined with other molecules in the form of hydrogen bonds and van der Waals forces rather than covalent bonds, so it is very easy to escape during use, and thus migrate to the environment and even the human body. Health hazards. [0003] Studies have shown that the absorption and distribution period of DEHP in the human body lasts for about 4 to 8 hours, most of which can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/12A23C13/16A23L19/20A23L33/00A61K35/744A61P39/02C12R1/01
CPCA61P39/02A23C9/12A23C13/16A23L19/20A23L33/00A61K2035/115A23V2002/00C12R2001/01C12N1/205A23V2400/231A23V2200/30A23B7/155A23B7/10A23C9/1234A23C19/032A23L33/135A23L29/065A61P39/00Y02A50/30A23C19/062A61K35/744A23C9/123A61K35/747C12N1/20A61K39/09
Inventor 王刚陈卫陈潜赵建新张灏
Owner 无锡食生臻选生物科技有限公司
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