Lactococcus lactis subsp. ccfm1018, its fermented food and its application in the preparation of medicine

A CCFM1018, Lactococcus lactis technology, applied in the field of microorganisms, can solve problems such as unsatisfactory adsorption effect, achieve good gastric acid resistance and bile salt resistance, reduce damage, and reduce the effect of content

Active Publication Date: 2020-04-14
无锡食生臻选生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing literature reports, the highest adsorption rate of probiotics to DEHP in vitro is only 9.62%, and the adsorption effect is not ideal.

Method used

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  • Lactococcus lactis subsp. ccfm1018, its fermented food and its application in the preparation of medicine
  • Lactococcus lactis subsp. ccfm1018, its fermented food and its application in the preparation of medicine
  • Lactococcus lactis subsp. ccfm1018, its fermented food and its application in the preparation of medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Lactococcus lactis subsp. lactis CCFM1018 has good tolerance to simulated gastrointestinal fluid

[0069] The cryopreserved Lactococcus lactis subsp. lactis CCFM1018 was streaked in a solid medium, incubated aerobic at 30°C for 24 hours, and then subcultured in liquid medium for 2 to 3 times. Culture medium of CCFM1018, centrifuged at 8000×g for 5 min to collect the bacteria, resuspended in (1:1) pH2.5 artificial simulated gastric juice (containing 1% pepsin, pH=2.5 MRS medium) mixed, and then at 37 ℃ Under aerobic culture, samples were taken at the beginning (0h), 1h, 2h and 3h respectively, and the plate colonies were counted by pouring and culturing with MRS agar medium, and the number of viable bacteria was determined and the survival rate was calculated. The survival rate is the ratio of the number of viable cells in the culture solution to the number of viable cells at 0 h, expressed in %.

[0070] Take the culture broth of Lactococcus lactis subsp. lactis ...

Embodiment 2

[0075] Example 2: Lactococcus lactis subsp. lactic acid CCFM1018 has good adsorption capacity for DEHP and MEHP in an aqueous solution containing plasticizer DEHP or MEHP in vitro

[0076] Bacterial adsorption: After purifying and cultivating the experimental bacteria, they are connected to MRS liquid medium according to 1% (v / v) inoculum and cultured at 30℃ for 20h (E.coli is cultured with LB medium at 37℃ with shaking; E. .faecalis was cultured in MRS liquid medium at 37°C), then centrifuged at 8000×g for 20 minutes, discarded the supernatant, resuspended in ultrapure water, and centrifuged at 8000×g for 20 minutes, discarded the supernatant. Live bacteria cells, that is, wet bacteria. Suspend the wet bacteria body weight in 50mg / LDEHP or 10mg / L MEHP aqueous solution, and make the final bacterial cell concentration reach 1g wet bacteria body / L. The blank control is the wet bacteria body weight suspension in ultrapure water without DEHP and MEHP. The volume of each group is 1 m...

Embodiment 3

[0083] Example 3: Lactococcus lactis subsp. lactis CCFM1018 has no acute side effects on SD rats

[0084] Resuspend Lactococcus lactis subsp. lactis CCFM1018 in a 2% (w / v) sucrose solution with a cell density of 1.0×10 9 CFU / mL. Take 10 healthy male SD rats with a weight of about 100g, give 2mL of this concentration suspension daily, observe for a week, and record the death and body weight.

[0085] These test results are listed in Table 4. The results show that the feeding concentration is 1.0×10 9 The CFU / mL Lactococcus lactis subsp. lactis CCFM1018 did not cause significant effects on the rats, the body weight did not change significantly, and there was no death. The rat has no obvious pathological symptoms.

[0086] Table 4 Changes in rat body weight and death

[0087]

[0088] Note: -: No rats died

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Abstract

The invention discloses Lactococcus lactis lactic acid subspecies CCFM1018, its fermented food and its application in preparing medicine. The effect of the bacteria on promoting the excretion of DEHP and MEHP is not only significantly better than the resident flora E. coli and Enterococcus faecalis, but also better than the commercial strain Lactobacillus rhamnosus LGG. Therefore, the Lactococcus lactis subsp. lactic acid CCFM1018 of the present invention can be used as an effective means to prevent and relieve body damage caused by DEHP and MEHP, and does not have the toxic and side effects of drugs. Lactococcus lactis subspecies lactic acid CCFM1018 can be used to prepare pharmaceutical compositions and fermented foods for alleviating and preventing the toxicity of DEHP and its metabolites, and has very broad application prospects.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to Lactococcus lactis subsp. lactis CCFM1018, its fermented food and its application in preparing medicines. Background technique [0002] As a plasticizer, bis(2-ethylhexyl) phthalate (DEHP) can be added to plastics to increase its flexibility and plasticity. Due to its low price, DEHP is widely used in the production of plastic products such as medical equipment and chemical products. It is currently the most widely used plasticizer in my country. In these plastic products, DEHP is mainly combined with other molecules in the form of hydrogen bonds and van der Waals forces rather than covalent bonds, so it is easy to escape during use, and thus migrate to the environment and even the human body. Health hazards. [0003] Studies have shown that the absorption and distribution period of DEHP in the human body lasts for about 4 to 8 hours, most of which can be completely ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/12A23C13/16A23L19/20A23L33/00A61K35/744A61P39/02C12R1/01
CPCA61P39/02A23C9/12A23C13/16A23L19/20A23L33/00A61K2035/115A23V2002/00C12R2001/01C12N1/205A23V2400/231A23V2200/30A23B7/155A23B7/10A23C9/1234A23C19/032A23L33/135A23L29/065A61P39/00Y02A50/30A23C19/062A61K35/744A23C9/123A61K35/747C12N1/20A61K39/09
Inventor 王刚陈卫陈潜赵建新张灏
Owner 无锡食生臻选生物科技有限公司
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