Lactococcus lactis subsp. ccfm1018, its fermented food and its application in the preparation of medicine
A CCFM1018, Lactococcus lactis technology, applied in the field of microorganisms, can solve problems such as unsatisfactory adsorption effect, achieve good gastric acid resistance and bile salt resistance, reduce damage, and reduce the effect of content
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Embodiment 1
[0068] Example 1: Lactococcus lactis subsp. lactis CCFM1018 has good tolerance to simulated gastrointestinal fluid
[0069] The cryopreserved Lactococcus lactis subsp. lactis CCFM1018 was streaked in a solid medium, incubated aerobic at 30°C for 24 hours, and then subcultured in liquid medium for 2 to 3 times. Culture medium of CCFM1018, centrifuged at 8000×g for 5 min to collect the bacteria, resuspended in (1:1) pH2.5 artificial simulated gastric juice (containing 1% pepsin, pH=2.5 MRS medium) mixed, and then at 37 ℃ Under aerobic culture, samples were taken at the beginning (0h), 1h, 2h and 3h respectively, and the plate colonies were counted by pouring and culturing with MRS agar medium, and the number of viable bacteria was determined and the survival rate was calculated. The survival rate is the ratio of the number of viable cells in the culture solution to the number of viable cells at 0 h, expressed in %.
[0070] Take the culture broth of Lactococcus lactis subsp. lactis ...
Embodiment 2
[0075] Example 2: Lactococcus lactis subsp. lactic acid CCFM1018 has good adsorption capacity for DEHP and MEHP in an aqueous solution containing plasticizer DEHP or MEHP in vitro
[0076] Bacterial adsorption: After purifying and cultivating the experimental bacteria, they are connected to MRS liquid medium according to 1% (v / v) inoculum and cultured at 30℃ for 20h (E.coli is cultured with LB medium at 37℃ with shaking; E. .faecalis was cultured in MRS liquid medium at 37°C), then centrifuged at 8000×g for 20 minutes, discarded the supernatant, resuspended in ultrapure water, and centrifuged at 8000×g for 20 minutes, discarded the supernatant. Live bacteria cells, that is, wet bacteria. Suspend the wet bacteria body weight in 50mg / LDEHP or 10mg / L MEHP aqueous solution, and make the final bacterial cell concentration reach 1g wet bacteria body / L. The blank control is the wet bacteria body weight suspension in ultrapure water without DEHP and MEHP. The volume of each group is 1 m...
Embodiment 3
[0083] Example 3: Lactococcus lactis subsp. lactis CCFM1018 has no acute side effects on SD rats
[0084] Resuspend Lactococcus lactis subsp. lactis CCFM1018 in a 2% (w / v) sucrose solution with a cell density of 1.0×10 9 CFU / mL. Take 10 healthy male SD rats with a weight of about 100g, give 2mL of this concentration suspension daily, observe for a week, and record the death and body weight.
[0085] These test results are listed in Table 4. The results show that the feeding concentration is 1.0×10 9 The CFU / mL Lactococcus lactis subsp. lactis CCFM1018 did not cause significant effects on the rats, the body weight did not change significantly, and there was no death. The rat has no obvious pathological symptoms.
[0086] Table 4 Changes in rat body weight and death
[0087]
[0088] Note: -: No rats died
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