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Swim bladder-derived biological valve material and its preparation method and application

A technology derived from organisms and swim bladders, applied in tissue regeneration, medical science, prostheses, etc.

Active Publication Date: 2021-04-20
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that carp swim bladder has been used to successfully repair rabbit dura mater and dog bladder, but so far there has been no report on using it as a material for heart valves

Method used

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  • Swim bladder-derived biological valve material and its preparation method and application
  • Swim bladder-derived biological valve material and its preparation method and application
  • Swim bladder-derived biological valve material and its preparation method and application

Examples

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preparation example Construction

[0026] The invention provides a method for preparing a swim bladder-derived biological valve material, comprising the steps of:

[0027] S1 (decellularization of swim bladder): fresh bony fish swim bladders were preserved in PBS solution containing penicillin and streptomycin, and then placed in ice packs for later use; among them, bony fish include silver carp, grass carp, carp, yellow croaker, and sturgeon One or more of fish, Spanish mackerel and totoaba; in the PBS solution containing penicillin and streptomycin, the concentration of penicillin is 100U / mL, and the concentration of streptomycin is 0.1mg / mL;

[0028] Under sterile conditions, cut the swim bladder of bony fish, rinse it several times with sterile PBS solution, and then perform decellularization treatment, including the steps of: placing the swim bladder in 1% SDS solution at room temperature for 6-24 hours, and then transferring Treat in 1% Triton X-100 at room temperature for 0-120min, then soak and rinse in...

Embodiment 1

[0032] This embodiment provides a method for preparing a biological valve material derived from silver carp swim bladder, comprising steps:

[0033] S1 (decellularization treatment of silver carp swim bladder): fresh silver carp swim bladder was preserved in PBS solution containing penicillin and streptomycin, and then placed in ice pack for later use; in the PBS solution containing penicillin and streptomycin, the concentration of penicillin 100U / mL, the concentration of streptomycin is 0.1mg / mL;

[0034] Under sterile conditions, cut the silver carp swim bladder, and then rinse it several times with sterile PBS solution; then perform decellularization, including the steps: place the silver carp swim bladder in 1% SDS solution for 6 hours at room temperature, and then transfer Treat in 1% Triton X-100 at room temperature for 30 minutes, then soak and rinse in PBS solution for 48 hours, and then put it in a solution containing 0.2 mg / mL DNase and 20 mg / mL RNase for overnight d...

Embodiment 2

[0037] This embodiment provides a method for preparing a biological valve material derived from grass carp swim bladder, comprising steps:

[0038] S1 (grass carp swim bladder decellularization treatment): fresh grass carp swim bladder was stored in a PBS solution containing penicillin and streptomycin, and then placed in an ice pack for later use; in the PBS solution containing penicillin and streptomycin, the concentration of penicillin was 100U / mL, the concentration of streptomycin is 0.1mg / mL;

[0039] Under sterile conditions, the aortic valve of the grass carp swim bladder was cut, and then rinsed several times with sterile PBS solution; and then the grass carp swim bladder was decellularized, including the steps of: placing the grass carp swim bladder in 1% SDS solution for 6 hours at room temperature , then transferred to 1% Triton X-100 at room temperature for 30 minutes, then soaked and rinsed in PBS solution for 48 hours, and then placed in a solution containing 0....

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Abstract

The invention relates to a swim bladder-derived biological valve material and its preparation method and application. The preparation method comprises the steps of: cutting the swim bladder and performing decellularization treatment on it to obtain the decellularized swim bladder; fixing and cross-linking the decellularized swim bladder, and the cross-linking is completed Afterwards, rinsing is carried out to obtain the swim bladder-derived biological valve material. Compared with the existing bovine pericardium-derived biological valve material, the swim bladder-derived biological valve material provided by the present invention is smooth on both sides, and the thickness of the material is good, and there is almost no fat and other adhesion tissues, so that The raw material processing process is easier to operate, and the yield of biological valves prepared from fish swim bladder is higher; the mechanical properties are equivalent to bovine pericardium-derived biological valve materials, but the anti-calcification performance is far superior to bovine pericardial materials, avoiding the short-lived life of bovine pericardial biological valves The defect solves the problems of poor durability and easy calcification of the existing bovine pericardium-derived biological heart valve.

Description

technical field [0001] The invention relates to the technical field of biological heart valves, in particular to a swim bladder-derived biological valve material and its preparation method and application. Background technique [0002] In the 1960s, biological valves emerged as the times require. They do not require long-term anticoagulant therapy and have excellent hemodynamic performance. In recent years, they have been used more and more in heart surgery valve replacement operations. Especially for elderly patients who are at risk of anticoagulation, biological valves are undoubtedly the best choice for heart valve replacement surgery. At the same time, as Transcatheter aortic valve replacement (TAVR) has become a hot new technology for valve treatment in the world, the artificial valve used in TAVR is a biological valve, which shows that the demand for biological valves will increase sharply in the future. surge. At present, most of the bioprosthetic valves used clinic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/36
CPCA61L27/3604A61L27/3687A61L27/50A61L2430/20A61L2430/40A61L2430/36
Inventor 王志红刘婧秦怡博冷希岗孔德领
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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