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A method for improving the transplanting survival rate of spring grass tissue culture seedlings

A technique for transplanting survival rate and spring grass is applied in the field of plant cultivation to achieve the effects of improving the transplanting survival rate, increasing the proliferation rate and improving the stability of offspring

Active Publication Date: 2021-06-29
ZHANGZHOU INST OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the tissue culture of Springgrass broadleaf at present, and there are only reports of using flower buds as explants for tissue culture and propagation

Method used

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  • A method for improving the transplanting survival rate of spring grass tissue culture seedlings
  • A method for improving the transplanting survival rate of spring grass tissue culture seedlings
  • A method for improving the transplanting survival rate of spring grass tissue culture seedlings

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Experimental program
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Effect test

Embodiment 1

[0027] 1 Culture medium preparation:

[0028] 1) Induction medium: MS ( where KH 2 PO 4 double )+6-BA 2.0mg / L+NAA 0.2mg / L+sucrose 30g / L+carrageenan 6.0g / L, pH=5.8

[0029] 2) subculture medium: MS ( where KH 2 PO 4 double )+6-BA 1.0mg / L+KT 1.0mg / L+NAA 0.2mg / L+sucrose 30g / L+carrageenan 6.0g / L, pH=5.8

[0030] 3) Rooting medium: 1 / 2MS ( where KH 2 PO 4 double )+IBA 1.0mg / L+KT 0.2mg / L+NAA 1.0mg / L+activated carbon 1.0g / L+sucrose 20g / L+carrageenan 6.0g / L, pH=5.8

[0031] 2. Selection and disinfection of explants:

[0032] Get diameter 2cm, be in dormant spring spring grass bulb as material, air in the shade for 8 days. Peel off its outer dry scales, cut off the roots, and rinse well. In the ultra-clean workbench, soak in 70% alcohol for 30s, then disinfect with 0.1% mercuric acid (add 1-2 drops of Tween-80) for 5-10min, rinse with sterile water for 4 times, and then clean it with sterile filter paper. Drain remaining water. Then, excise the scales at the upper e...

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Abstract

The invention discloses a method for improving the transplanting survival rate of spring grass tissue culture seedlings. The invention uses plant tissue culture technology to induce regeneration of spring grass bulbs, and carries out subculture, rooting culture and subsequent refinement. Transplantation of seedlings improves offspring stability, shortens induction period, increases proliferation rate, and improves transplant survival rate.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for improving the transplanting survival rate of spring grass tissue culture seedlings. Background technique [0002] Broad-leaf spring grass (Ornithogalum concodianum) is a perennial ornamental succulent plant belonging to the Liliaceae tiger eye Dieffenbachia. It has round or irregular bulbs, the surface of the underground part is yellowish white, and the part exposed to the soil surface is greenish white after being exposed to the sun. The fleshy leaves emerge from the top of the bulb and grow upright at first, then twist and spiral. They are mostly produced in the desert areas of southern Africa, and South Africa is the main producing area. As a rare flower, spring grass grows slowly under natural conditions, has a low reproduction rate, and is dormant in summer. At present, the quantity in China is scarce and the price is high, and the price...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈汉鑫余德亿万学锋黄鹏
Owner ZHANGZHOU INST OF AGRI SCI