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Suspension beads array system for detecting coronary heart disease susceptibility gene site

A susceptibility gene, coronary heart disease technology, applied in the field of suspended microbead array system, can solve the problems of low frequency of occurrence, difficult to explain the high incidence of coronary heart disease, and achieve the effect of reducing costs and medical costs

Inactive Publication Date: 2018-10-19
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that individual mutations in certain genes can lead to different types of coronary heart disease. The frequency of such mutations is often extremely low, and it is difficult to explain the high incidence of coronary heart disease.

Method used

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  • Suspension beads array system for detecting coronary heart disease susceptibility gene site
  • Suspension beads array system for detecting coronary heart disease susceptibility gene site
  • Suspension beads array system for detecting coronary heart disease susceptibility gene site

Examples

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Effect test

Embodiment 1

[0022] Example 1. Construction of Suspension Microbead Array System for Coronary Heart Disease Susceptibility Gene Loci

[0023] 1. Multiplex PCR primer design

[0024] In order to realize the detection of the five coronary heart disease susceptibility loci, a total of five PCR amplifications are required. The PCR primer sequences corresponding to the five CHD gene susceptibility loci are shown in Table 1.

[0025] Table 1. Multiplex PCR Primer Sequences

[0026]

[0027] 2. Probe design

[0028] In the present invention, 10 probes are designed in total, that is, one normal probe (N) and one mutant probe (M) are designed for each gene locus, and the sequences of the 10 probes are shown in Table 2.

[0029] Table 2. Probe sequences

[0030]

[0031] ,

[0032] Among them, rs6903956-N, rs2123536-N, rs1842896-N, rs9268402-N and rs7136259-N represent the normal probes of each locus; Variant probes for each locus.

[0033] 3. Preparation of suspension microbead array...

Embodiment 2

[0067] Embodiment 2 Application of the suspended microbead array system of the present invention

[0068] Using the suspended microbead array coronary heart disease susceptibility gene locus detection method described in the present invention, based on the MagPix suspended microbead array reading instrument of Luminex Company, the results of the detection of 24 clinical samples are as follows:

[0069]

[0070] Compared with the results of the 5 susceptibility loci in these samples, the accuracy rate was 100% compared with the classical Sanger sequencing method.

[0071] The advantages of the suspension microbead array system described in the present invention to detect the susceptibility loci of coronary heart disease include: 1. the probes on the microbeads and the PCR products to be tested can be in the liquid suspension flow state, so the results are stable; 2. the experiment The cycle is short, and the entire detection process can be completed within 6 hours; ③The degr...

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Abstract

The invention relates to a suspension beads array system for detecting a coronary heart disease susceptibility gene site. The system realizes para-position detection of an rs6903956 site in a 6p24.1 segment, an rs2123536 site in a 2p24.1 segment, an rs1842896 site in a 4p32.1 segment, an rs9268402 site in a 6p21.32 segment and an rs7136259 site in a 12p21.33 segment in one experiment. The described suspension beads array system can finish detection of five sites in one experiment, and clinic targeted treatment is facilitated; and the cost of reagent consumables is greatly reduced by being shared to detection of single site, and thus the medical cost can be reduced.

Description

technical field [0001] The invention relates to a system for detecting susceptibility gene loci of coronary heart disease, in particular to a suspended microbead array system for detecting susceptibility gene loci of coronary heart disease. Background technique [0002] Congenital heart disease is an abnormality in the shape, structure, and function of the heart and blood vessels caused by the disorder of the heart and blood vessels during embryonic development. The incidence rate of congenital heart disease is 0.8%, ranking first in my country for four consecutive years. Patients with congenital heart disease usually need to receive long-term treatment, causing great economic burden and mental pressure to patients, families and society. Most of the causes of coronary heart disease are the result of a combination of genetic and environmental factors. Studies have shown that individual mutations in certain genes can lead to different types of coronary heart disease. The freq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6827C12Q1/6837
CPCC12Q1/6827C12Q1/6837C12Q1/6883C12Q2600/156C12Q2531/113C12Q2537/143C12Q2563/107
Inventor 任建峰黄姣刘姣
Owner SHANGHAI OCEAN UNIV
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