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Diagnosis reagent for primary hepatocellular carcinoma, reagent kit and prevention and cure medicaments

A hepatocellular carcinoma, primary technology, applied in the direction of drug combination, gene therapy, anti-tumor drugs, etc., can solve the problems of difficult diagnosis, early diagnosis and prevention of hepatocellular carcinoma, improve survival rate and broad application prospects Effect

Inactive Publication Date: 2013-04-17
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the current primary hepatocellular carcinoma is difficult to diagnose, especially difficult to early diagnosis and prevention

Method used

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  • Diagnosis reagent for primary hepatocellular carcinoma, reagent kit and prevention and cure medicaments
  • Diagnosis reagent for primary hepatocellular carcinoma, reagent kit and prevention and cure medicaments
  • Diagnosis reagent for primary hepatocellular carcinoma, reagent kit and prevention and cure medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preliminary determination of the relationship between human phosphoglycerate mutase 1 and the occurrence and development of primary hepatocellular carcinoma

[0042] 1. Cell source and culture conditions

[0043] Human liver cancer cell line HepG2 was purchased from ATCC (American Type Culture Collection), and human normal liver cell line L02 was purchased from China Cell Culture Center.

[0044] HepG2 was cultured in DMEM, wherein 10% fetal bovine serum, 10 7 U / L penicillin and 10mg / L streptomycin, while L02 was cultured in RPMI 1640. The culture conditions were all 37°C, 5% CO 2 concentration.

[0045] 2. Reagent source

[0046] Cell culture stable isotope labeling technology (SILAC) related reagents: fetal bovine serum was purchased from Hyclone Company;

[0047] SDS-PAGE related reagents: DC protein assay kit was purchased from Bio-Rad; Coomassie Brilliant Blue R-250 was purchased from Merck;

[0048] Reagents related to enzymatic hydrolysis and mass s...

Embodiment 2

[0080] Example 2 Verification that phosphoglycerate mutase 1 is a protein related to the occurrence and development of primary hepatocellular carcinoma

[0081] RT-PCR and Western Blot were used to verify the expression difference of human phosphoglycerate mutase 1 between primary hepatocellular carcinoma and normal liver tissue at the transcriptional and translational levels.

[0082] 1. Sources of primary hepatocellular carcinoma and normal liver tissue samples

[0083] The tissue samples of 54 cases of primary hepatocellular carcinoma selected in this example were collected from patients with primary hepatocellular carcinoma undergoing surgery in the Department of Hepatobiliary Surgery, West China Hospital, Sichuan University, from April to June 2007. The age distribution of the patients ranged from 34 to 66 years old, with an average age of 44.8±8.5 years old. All patients had not received chemotherapy or radiotherapy before operation. The postoperative pathological diagno...

Embodiment 3

[0128] Example 3 Immunohistochemical analysis of the expression of human phosphoglycerate mutase 1 in primary hepatocellular carcinoma and normal liver tissue

[0129] 1. Tissue sample source

[0130] Paraffin specimens were obtained from the Department of Hepatobiliary Surgery, West China Hospital, Sichuan University. The relevant information of 54 cases of primary hepatocellular carcinoma tissue samples and 21 cases of normal liver tissue samples has been described in Example 2.

[0131] 2. Reagent source

[0132] EnVision + horseradish peroxidase (HRP) immunohistochemical staining system was purchased from Dako Cytomation GmbH in Hamburg, Germany, which included peroxidase blocking solution (Peroxidase Block), horseradish peroxidase-labeled polymer conjugated sheep antibody Rabbit IgG (Labelled Polymer-HRP ANTI-RABBIT), substrate buffer (Substrate Buffer), DAB staining solution (DAB+Chromogen). Goat anti-human phosphoglycerate mutase 1 antibody was purchased from Abcam. ...

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Abstract

The present invention belongs to a field of diagnosing, preventing and curing knub, particularly relates to a diagnosis reagent for primary hepatocellular carcinoma, a reagent kit and prevention and cure medicaments. The invention provides a novel diagnosis reagent and medicaments for the early diagnosis and prevention and cure of the primary hepatocellular carcinoma, also provides a new method for selecting medicaments for the prevention and cure of the primary hepatocellular carcinoma. The diagnosis reagent contains a reagent for detecting a transcription level or a translation level of human phosphoglyceromutase 1 gene. The diagnosis reagent may be used for the early diagnosis of the primary hepatocellular carcinoma and has a good application prospect. The medicaments for the preventionand cure of the primary hepatocellular carcinoma is that a medicament capable of changing the transcription level or the translation level of the human phosphoglyceromutase 1 gene and representing anexpression carrier of shRNA molecule of the human phosphoglyceromutase 1, etc.

Description

technical field [0001] The invention belongs to the field of tumor diagnosis and prevention, and in particular relates to a primary hepatocellular carcinoma diagnostic reagent, kit and prevention and treatment medicine. Background technique [0002] Primary hepatocellular carcinoma is one of the most common malignant tumors in my country, ranking third in digestive system malignant tumors, second only to gastric cancer and esophageal cancer. About 300,000 people die from primary hepatocellular carcinoma every year in my country, accounting for 50% of the world's liver cancer deaths. It is worth noting that the incidence of primary hepatocellular carcinoma is on the rise all over the world. The main clinical manifestation of primary hepatocellular carcinoma is distending pain or dull pain in the liver area, and jaundice will appear in the late stage. The clinical diagnosis mainly depends on the clinical manifestations and the concentration of serum alpha-fetoprotein, but cas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/574G01N33/577A61K48/00A61P35/00A61P1/16
Inventor 黄灿华魏于全任凤莲吴泓
Owner SICHUAN UNIV
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