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Method for separating and purifying tanshinone IIA from radix salviae miltiorrhizae by using polyamide gel

A technology for tanshinone and polyamide, applied in the field of separation and purification of tanshinone IIA in Salvia miltiorrhiza, can solve the problems of high cost, low efficiency, low content and the like, and achieve the effects of low cost, high yield and purity, and small residual solvent degree

Inactive Publication Date: 2018-11-02
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The content of tanshinone IIA in Salvia miltiorrhiza is low, and conventional organic reagent extraction and silica gel column separation technology are used, which has low efficiency and high cost

Method used

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  • Method for separating and purifying tanshinone IIA from radix salviae miltiorrhizae by using polyamide gel
  • Method for separating and purifying tanshinone IIA from radix salviae miltiorrhizae by using polyamide gel
  • Method for separating and purifying tanshinone IIA from radix salviae miltiorrhizae by using polyamide gel

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Embodiment 1

[0020] Embodiment 1: Salvia miltiorrhiza active ingredient extraction

[0021] 1.1 Smash

[0022] Wash 10kg of salvia miltiorrhiza root material, dry naturally, and pulverize.

[0023] 1.2 Ethanol immersion extraction

[0024] Add 16L of 95vol% ethanol to soak for 5-7 days, stirring once every 12 hours during this period; filter the extract through a sand core funnel, take the liquid and evaporate the solvent with a rotary evaporator, and recover the extract; pour the solvent back into the material Soaking and extracting is repeated 3 to 5 times, and finally the filtrates are combined to obtain the salvia miltiorrhiza extract.

[0025] 1.3 Petroleum ether extraction

[0026] Pour the obtained ethanol extraction extract into a separatory funnel, first add 5L of pure water, then add 2L of petroleum ether to fully shake, wait for standing to separate layers, take the upper layer of petroleum ether phase, distill off the solvent, repeat the above operation for 3~ 5 times to ob...

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Abstract

The invention relates to a method for separating and purifying tanshinone IIA from radix salviae miltiorrhizae by using polyamide gel, which belongs to the technical field of the development of traditional Chinese medicines. The method adopts petroleum ether and vinyl acetate as extraction agnets, utilizes a polyamide gel column to separate extract rich in a target substance, and utilizes ethanolto perform gradient elution separation to obtain a compound tanshinone IIA, wherein the extraction separation purity is about 93 percent. The extraction process is less in solvent residue, safe and reliable; and by utilizing the polyamide gel, the cost is relatively low, the yield and purity of the obtained tanshinone IIA are relatively high, and the method is an efficient method for extracting and separating the tanshinone IIA, and provides scientific evidence for reasonably utilizing the separation extraction technology of the tanshinone IIA.

Description

technical field [0001] The invention relates to a method for separating and purifying tanshinone IIA in salvia miltiorrhiza, belonging to the technical field of traditional Chinese medicine development. Background technique [0002] Salvia miltiorrhiza Bunge is a perennial herb of the genus Salvia in the family Lamiaceae. It is named "Salvia miltiorrhiza Bunge" because its root color is red and its shape resembles ginseng. It is a commonly used traditional Chinese medicine for treating cardiovascular and cerebrovascular diseases. The active ingredients of Danshen mainly include water-soluble phenolic acid compounds and fat-soluble tanshinone compounds. Tanshinone compounds mainly include tanshinone I, tanshinone IIA, tanshinone IIB, cryptotanshinone, dihydrotanshinone, etc. In recent years, studies have found that tanshinone IIA can inhibit the growth of various tumor cell lines including breast cancer, lung cancer, and liver cancer; it plays an important role in improving ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00
CPCC07J73/003
Inventor 开国银张毅时敏黄国正邓昌平付蓉王瑶
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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