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Method for identifying pumpkin bacterial wilt resistant varieties in greenhouse seedling stage

A technology for bacterial wilt and pumpkin, applied in the field of identification of resistant varieties of pumpkin bacterial wilt at the seedling stage in the greenhouse, to achieve the effects of uniform disease incidence, reduced time required, and consistent results

Inactive Publication Date: 2018-11-06
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports of pumpkin varieties with high resistance to pumpkin bacterial wilt in my country, and a fast, stable and reliable method for identifying resistant varieties must be established for screening resistant varieties

Method used

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  • Method for identifying pumpkin bacterial wilt resistant varieties in greenhouse seedling stage
  • Method for identifying pumpkin bacterial wilt resistant varieties in greenhouse seedling stage
  • Method for identifying pumpkin bacterial wilt resistant varieties in greenhouse seedling stage

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] 1. Test materials

[0028] Taking 3 pumpkin varieties (pocket honeybeen squash, honeybeen squash and big fruit honeybeen squash) as the research object, different pumpkin varieties were treated by root soaking inoculation method and injection inoculation method, and the bacterial wilt disease of pumpkin varieties after inoculation with different methods was analyzed. Incidence differences.

[0029] 2. Test method

[0030] 1. Preparation of inoculated bacteria

[0031] The Ralstonia solanacearum strain (RS378; doi:10.3389 / fpls.2017.01794) stored at 20°C, that is, references: X She, L Yu, G Lan, Y Tang, Z He. Identification and Genetic Characterization of Ralstonia solanacearum Species Complex Isolates from Cucurbita maxima in China. "Frontiers in Plant Science", 2017, 8:1794). Activate on the TZC plate, culture at 30°C for 48 hours, then pick a single colony and inoculate it on the slant of the TZC medium test tube, cultivate it at 30°C for 24 hours, then pick fresh c...

Embodiment 2

[0050] 1. Test materials

[0051] Taking a medium-resistant pumpkin cultivar (Indian pumpkin) as the research object, its resistance to pumpkin bacterial wilt was identified by root soaking inoculation method.

[0052] 2. Test method

[0053] 1. Preparation of inoculated bacteria

[0054] Activate the Ralstonia solanacearum strain (RS378) stored at 20°C on a TZC plate, culture it at 30°C for 48 hours, then pick a single colony and inoculate it on the slant of the TZC medium test tube, and culture it at 30°C for 24 hours , and then pick fresh colonies from the slant of the TZC medium test tube and inoculate them into NA liquid medium, cultivate them at 150rpm / min at 30°C for 24 hours, and prepare the RS378 bacterial solution with water to a concentration of 10 8 ~10 9 The suspension of CFU / ml is ready for use.

[0055] 2. Material preparation for test pumpkin varieties

[0056] Cultivate pumpkin seedlings for inoculation: 72 hole seedling trays (54cm × 28cm, hole depth 4cm...

Embodiment 3

[0072] 1. Experimental materials

[0073] Taking 11 pumpkin cultivar materials as the research object, the resistance to pumpkin bacterial wilt was identified by root soaking inoculation method.

[0074] 2. Test method

[0075] 1. Preparation of inoculated bacteria

[0076] Activate the Ralstonia solanacearum strain (RS378) stored at 20°C on TZC plates, culture at 30°C for 48 hours, pick a single colony and inoculate it on the slant of the TZC medium test tube, and culture at 30°C for 24 hours , and then pick fresh colonies from the slant of the TZC medium test tube and inoculate them into NA liquid medium, cultivate them at 150rpm / min at 30°C for 24 hours, and prepare the RS378 bacterial solution with water to a concentration of 10 8 ~10 9 The suspension of CFU / ml is ready for use.

[0077] 2. Material preparation for test pumpkin varieties

[0078] A 72-hole seedling tray (54cm×28cm, hole depth 4cm, hole opening diameter 3.8cm, hole bottom diameter 2.2cm) was surface-st...

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Abstract

The invention discloses a methodfor identifying pumpkin bacterial wilt resistant varieties in the greenhouse seedling stage. The method comprises the steps of (1) cultivating pumpkin seedlings for inoculation, wherein firstly 2-3 the pumpkin seedlings for inoculation in the true leaf stage are cultivated in a greenhouse; (2) inoculating, whereinpumpkin ralstonia solanacearum is inoculated on the pumpkin seedlings for inoculation by a root soaking inoculation method; (3) conducting an investigation method, whereininvestigation is conducted once a week after inoculation, the total number of plants and the number of diseased plants after inoculation of the pumpkin ralstonia solanacearum are recorded, the diseased plant rate is counted, andthe resistance of the pumpkin varieties to pumpkin bacterial wilt is judged. The method has simple operation, short time required and accurate and reliable identification result, is not affected by the field environment, can significantly distinguish thedifference in resistance of different pumpkin varieties to the bacterial wilt, is suitable for the identification and screeningof resistance of large quantities of pumpkin materialsto the bacterial wilt simultaneously, and is beneficial to improving the screening efficiency of disease-resistant materials.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to a method for identifying pumpkin bacterial wilt resistance varieties at the greenhouse seedling stage. Background technique [0002] Pumpkin bacterial wilt is a soil-borne vascular disease caused by R. Pumpkin bacterial wilt is a newly discovered disease in the south in recent years, and the rate of diseased plants in the field is about 25-45%, causing serious economic losses. Because pumpkin R. solanacearum can survive in the soil for a long time, it is difficult to control pumpkin bacterial wilt. [0003] At present, the control measures for pumpkin bacterial wilt mainly include cultivation measures such as crop rotation with non-hosts and Solanaceae crops, adjusting soil acidity, and timely pulling out diseased plants, but the control effect is not ideal; using fungicides such as 77% copper hydroxide can Wet powder 1000 times liquid, or 1000 times liquid of 20% Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/05A01G31/00A01G7/00
CPCA01G7/00A01G22/05A01G31/00
Inventor 佘小漫何自福汤亚飞蓝国兵于琳李正刚邓铭光
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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