Dendrimer transgene carrier modified with bone-targeting peptide and naphthalimide, preparation method and application thereof

A dendrimer and transgenic vector technology, applied in the fields of polymer chemistry and biological materials, can solve the problems of low transfection efficiency, lack of targeting groups, poor cell selectivity, etc., and achieve high transfection efficiency, cheap and easy raw materials. high-efficiency, low cytotoxicity
CN108753829BActive Publication Date: 2021-06-29西安九清医疗科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
西安九清医疗科技有限公司
Publication Date
2021-06-29

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Abstract

The present invention provides a dendrimer transgene carrier modified with bone-targeting peptide and naphthalimide, which includes a dendrimer skeleton, a bone-targeting peptide group, and a functional group containing naphthalimide. The targeting peptide group and the naphthalimide functional group are covalently bonded on the surface of the dendrimer; the dendrimer is a polyamide-amine dendrimer. The invention also provides the preparation method of the gene transfection carrier and the application of the dendrimer transgene carrier as a nucleic acid molecule delivery carrier. The invention adopts a brand-new modification method and a functional group to modify the dendrimer, the reaction is simple and efficient, the yield is high, the efficient transfection effect is achieved in the cell transfection process, and the bone targeting performance is better. The production of materials is simple and low in cost, the cytotoxicity of the transfection process is low, and gene molecules can be effectively and safely transported into cells. The dendrimer transgene carrier of the present invention has the advantages of high efficiency, low toxicity, low price, and simple synthesis .
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Description

technical field

[0001] The invention belongs to the technical field of polymer chemistry and biomaterials, and in particular relates to a dendrimer transgene carrier modified with a bone-targeting peptide and naphthalimide, a preparation method and application thereof. Background technique

[0002] As a new type of treatment, gene therapy has shown great application potential in the treatment of major diseases such as cancer, AIDS, and osteoporosis. However, due to the lack of safe and efficient targeted gene carriers, there has been no breakthrough in gene therapy for bone diseases such as osteoporosis.

[0003] Gene therapy refers to the introduction of exogenous normal genes into target cells by means of gene carriers to correct or compensate diseases caused by gene defects or abnormalities, so as to achieve the purpose of treatment. However, naked DNA generally exists in the form of a stretched linear helix with a loose volume, and the DNA molecule itself is a negativel...

Claims

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