Screening method for beer components that affect human satiety
A technology of satiety and beer, applied in the field of beer, can solve problems such as difficult analysis, inability of brewers to provide theoretical basis and measures for improving beer drinkability, unclear material basis of beer gastric emptying and diuretic difference, etc. , to achieve the effect of reducing satiety and improving beer drinkability
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Embodiment 1
[0040] 1. Gastric emptying analysis of 26 beer samples (including non-alcoholic beer, 8-12 degree Lager beer, fruit beer, ALE beer):
[0041] Preparation of activated carbon suspension: Accurately weigh 10 g of gum arabic powder, add 80 mL of distilled water, boil until transparent; add 10 g of activated carbon powder, boil three times, and set aside.
[0042] Animal preparation: Adult Kunming male mice were observed for 1 week before the test, and those with clinical abnormalities were excluded from the test.
[0043] After the mice were weighed, they were gavaged with beer to test their gastric emptying rate: gavage with a dose of 0.4mL beer + 0.1mL carbon powder / 25g (800mL / 50Kg body), and the mice were killed by cervical dislocation after 20 minutes of gavage. Open the abdominal cavity, quickly separate the gastrointestinal tract, observe the traveling route of the activated carbon, and calculate the propulsion rate of the carbon powder. The formula for calculating the end...
Embodiment 2
[0070] 1. Gastric emptying analysis of 30 beer samples:
[0071] The analysis method is the same as in Example 1, except that the mice are adult Kunming female mice.
[0072] 2. Determination of beer components:
[0073] Assay method is the same as in Example 1.
[0074] 3. Screening of beer components that affect human satiety:
[0075] Establish the PLSR regression equation between beer components and satiety, and use the absolute value of the standardized coefficient SRC to determine the possibility of affecting human satiety Beer components, ethanol, isoamyl alcohol, acetic acid, succinic acid, citric acid, K + 、Na + , more than tetrasaccharide, maltotriose, ethyl octanoate, lysine and tyrosine (12), the SRC value of each component is shown in Table 6;
[0076] The SRC value of each component in table 6 beer
[0077] components standardized coefficient ethanol 0.48 Isoamyl alcohol 0.43 Acetic acid -0.38 Succinic acid 0.46 citric...
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