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Digital protein quantification

A protein, high-throughput technology that can be used in measurement devices, microbial determination/inspection, instruments, etc., and can solve problems such as expensive

Pending Publication Date: 2018-11-09
BIO RAD LAB INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Methods such as mass cytometry require expensive and specialized heavy atom labeling to work and are limited to dozens of simultaneous markers for all but the most experienced users

Method used

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  • Digital protein quantification

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Embodiment Construction

[0039] I. Overview

[0040] Described herein are compositions, methods and kits for quantitative analysis of target biological components. The compositions, methods and kits described herein can be used to determine the levels of multiple target biological components in multiple cells at single cell resolution. The compositions, methods and kits described herein are based on the principle that levels of target biological components can be encoded into oligonucleotides. The oligonucleotide to be encoded is coupled to a binding element specific for the target biological component. The conjugated oligonucleotide includes a target ID sequence corresponding to the target biological component. The encoding may be by a binding event between the target biological component and the binding element. Detection of the target ID sequence of the encoded oligonucleotide enables detection of the target biological component, if present. High-throughput sequencing of multiple encoded oligon...

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Abstract

Methods and compositions are described for single cell resolution, quantitative proteomic analysis using high throughput sequencing.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 273,249, filed December 30, 2015, which is incorporated herein by reference in its entirety for all purposes. Background technique [0003] Next-generation sequencing methods have allowed quantitative analysis of thousands of nucleic acid markers at the whole organism to single-cell level. The difference is that quantification of other biological components, such as proteins, has proven much more difficult. Methods such as FACS, ELISA, and bead-based multiplexing are limited by lack of sensitivity, sample throughput, and the number of markers that can be analyzed simultaneously. Methods such as mass cytometry require expensive and specialized heavy atom labels to work and are limited to tens of simultaneous labels for all but the most experienced users. Contents of the invention [0004] Described herein are methods and compositions for th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6874G01N33/68
CPCC12Q1/6816C40B20/04C40B70/00C12Q1/6869G01N33/6878C12Q2563/159C12Q2565/514B01J19/0046
Inventor J·阿格雷丝迪R·雷伯弗斯基
Owner BIO RAD LAB INC
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