Preparation method of natamycin produced by fermentation regulation of Streptomyces gilvosporeus

A technology of Streptomyces chlorosporum and natamycin, applied in the field of bioengineering, can solve problems such as the effect of Streptomyces chlorosporium has not yet been found, and achieve the effects of reducing production cost and increasing yield

Inactive Publication Date: 2018-11-16
XIAMEN UNIV
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  • Preparation method of natamycin produced by fermentation regulation of Streptomyces gilvosporeus
  • Preparation method of natamycin produced by fermentation regulation of Streptomyces gilvosporeus
  • Preparation method of natamycin produced by fermentation regulation of Streptomyces gilvosporeus

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Effects of Adding Different Phytohormones at 0h on the Growth of Streptomyces chrysosporium and the Production of Natamycin

[0025] After Streptomyces luteus was activated on the plate, the spores of the activated strain were picked with an inoculation loop and inserted into a 250mL Erlenmeyer flask containing 50mL of seed medium, and cultured at 28°C and 200rpm for 24-48h. The activated strain was cultured in a 250mL shake flask with a carrier liquid volume of 50mL, and 1mg / LIAA, GA and 6-BA were added to the fermentation medium, cultured at 28°C and 200rpm. During the cultivation process, a sample was taken every 24h, and the biomass and natamycin production of each experimental group were measured. The results were as follows: Figure 1~3 shown.

[0026] Depend on figure 1 It can be seen that adding three kinds of phytohormones at 0h has no significant effect on promoting the growth of bacterial cells, but the experimental group adding 6-BA is more conducive to th...

Embodiment 2

[0029] Effects of adding 1mg / L 6-BA compound at 0h and adding 0.6% sodium propionate for 24h on the fermentation of Streptomyces chrysosporium

[0030] On the basis of Example 1, the experiment further compounded and added 0.6% sodium propionate at 24 hours, and investigated the effects of adding 1 mg / L6-BA compounded at 0 hours and adding 0.6% sodium propionate for 24 hours on the growth of Streptomyces chrysosporium cells and the accumulation of natamycin In order to further improve the output of natamycin, the shake flask fermentation was cultured to 72, 96 and 120h when samples were taken respectively, and the biomass and natamycin output of each experimental group were measured. The results are as follows Figure 4~6 shown.

[0031] Depend on Figure 4 It can be seen that adding the precursor substance sodium propionate on the basis of adding 6-BA can promote the growth of bacterial cells, and the 96h biomass is 33.0g / L, which is 13.8% higher than that of the control gro...

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Abstract

The invention provides a preparation method of natamycin produced by fermentation regulation of Streptomyces gilvosporeus and relates to the technical field of bioengineering. The method comprises steps as follows: strain activation, primary seed culture and shake flask fermentation, wherein the strain is Streptomyces gilvosporeus, ATCC 13326. The influence of three different plant hormones including IAA, GA and 6-BA, as well as precursor substance sodium propionate added in a fermentation process in a compounding manner on Streptomyces gilvosporeus cell growth and natamycin synthesis are investigated in order to understand the regulating effect of the plant hormones and the precursor substance on Streptomyces gilvosporeus cell growth and natamycin synthesis, and experimental evidence is provided for improving the efficiency of Streptomyces gilvosporeus fermentation based natamycin production by the plant hormones and the precursor substance.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a preparation method for regulating the fermentative production of natamycin by Streptomyces chrysosporium. Background technique [0002] In the food industry, microbial contamination will bring great harm to the production and preservation of products. For example, mycomycin produced after food is contaminated by fungi has pathogenicity such as hemorrhage, teratogenicity, and carcinogenicity, and will cause harm to human health. In order to solve this problem, people began to introduce food preservatives. At present, the production methods of food preservatives mainly include chemical synthesis and biotechnology, and the low-toxicity, broad-spectrum and economical and practical natural biological preservatives produced by biotechnology are gradually favored by people (Iraj Rasooli.et al.2007 ; Zhang JL. et al. 2010). [0003] Natamycin is a polyene macrolide compound wi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/62C12R1/465
CPCC12N1/20C12P19/626
Inventor 卢英华潘雪珊凌雪萍李俊陈雅萍陈翠雪邵文尧段然
Owner XIAMEN UNIV
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