Microorganism calcareous glue, preparation method and application thereof, sandy soil column and preparation method thereof

A technology of microorganisms and microbial bacteria liquid, applied in the fields of microbial calcareous glue and preparation, application, sand column and preparation, can solve the problem of high brittleness of the solidified sand column, and achieve the improvement of cohesion, contact force, and increase in The effect of a large angle of internal friction

Inactive Publication Date: 2018-11-16
INNER MONGOLIA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defect of high brittleness in the solidified sand column of the existing microorganism-induced calcium carbonate precipitation technology, the present invention proposes a green microbial calcium gel for fixing sand, a preparation method of the green micro

Method used

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  • Microorganism calcareous glue, preparation method and application thereof, sandy soil column and preparation method thereof
  • Microorganism calcareous glue, preparation method and application thereof, sandy soil column and preparation method thereof
  • Microorganism calcareous glue, preparation method and application thereof, sandy soil column and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0049] This embodiment provides a green microbial calcareous glue, which is mainly used for sandy soil consolidation. Knot.

[0050] The main components of the green microbial calcareous gel are mineralized microbial bacterial liquid, calcium source nutrient solution, and green colloid, mineralized microbial bacterial liquid, calcium source nutrient solution, and green gelatin. The volume ratio of the three is 1: (10-100): (0.001-1).

[0051] When the microbial bacterial liquid was prepared, the strain of the microbial bacterial liquid was Bacillus sarcina pasteurii (Sporosarcina pasteurii, ATCC11859), and the bacillus sarcina pasteurii was inoculated to the The culture medium was shaken and expanded to make a bacterial liquid.

[0052] Among them, the medium components are: 10~30g / L yeast powder, 2~10g / L NH 4 SO 4 , 0~15.748g / LC 4 h 11 NO 3 , 0~3g / L NiC l2 ; Remove the original medium from the bacterial solution after shaking and expansion, and then dilute it with fre...

Embodiment 2

[0059] This embodiment provides the preparation method of the green microbial calcareous gel of embodiment 1. The preparation method of the green microbial calcareous gel comprises the following steps.

[0060] Step 1, prepare the microbial bacterial liquid of mineralization performance

[0061] The strain of the microbial bacterial liquid is Bacillus sarcinis, and the bacillus sarcinis is inoculated into the culture medium according to the inoculum amount of 5%, and the culture medium is shaken and propagated to make the bacterial liquid, wherein, the culture medium The ingredients are: 10~30g / L yeast powder, 2~10g / L NH 4 SO 4 , 0~15.748g / L C 4 h 11 NO 3 , 0~3g / L NiC l2 .

[0062] Remove the original medium from the bacterial solution after shaking and expansion, and then dilute it with fresh medium, when the OD of the bacterial solution is 600 When the value is 0.5-1.0, the dilution is stopped, and the obtained bacterial liquid is the microbial bacterial liquid. The...

Embodiment 3

[0068] This embodiment discloses the method for using the green microbial calcareous glue in embodiment 1 in consolidating sandy soil, which includes the following steps.

[0069] Step 1: Prepare the microbial bacterial liquid with mineralization performance: the strain of the microbial bacterial liquid with mineralization performance is Bacillus pasteurii (Sporosarcina pasteurii, ATCC11859), which is inoculated into the culture medium according to the inoculum size of 5% and shaken Expand culture to make bacterial liquid, in which the medium components are 10-30g / L yeast powder, 2-10g / L NH 4 SO 4 , 0~15.748g / L C 4 h 11 NO 3 , 0~3g / L NiCl 2 , the bacterial solution after shaking and expansion was centrifuged in a centrifuge at 30°C and 200rpm for 20 minutes, the original medium was removed, and the Bacillus sarcina was diluted with fresh medium to remove the original medium. OD 600value (the absorbance value of the solution at a wavelength of 600nm, which is often used t...

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Abstract

The invention discloses environment-friendly microorganism calcareous glue, a preparation method and application thereof, a sandy soil column and a preparation method thereof. The environment-friendlymicroorganism calcareous glue is prepared from a microorganism bacteria solution with mineralization performance, a calcium source nutrient solution and an environment-friendly colloid in a volume ratio of 1 to (10 to 100) to (0.001 to 1). In the preparation of the microorganism bacterial solution, a culture medium is inoculated with 5 percent of sporosarcina pasteurii serving as a strain for performing oscillation and propagation to obtain a bacteria solution; in preparation of the calcium source nutrient solution, the calcium source nutrient solution is prepared according to a formula, andthe preparation of the nutrient solution is finished no less than 3 hours and no more than 6 hours before spraying. The environment-friendly colloid liquid of biomacromolecule liquid with colloidalityis prepared by dissolving exopolysaccharide of cell secretion or purified polysaccharide products. By adopting the environment-friendly microorganism calcareous glue, the defect of large brittlenessof a calcite cementation sand material by only relying on microorganism induction is overcome, and the concretion result of sand is effectively improved.

Description

technical field [0001] The invention relates to a green ecological consolidation method of sandy soil, specifically, a green microbial calcareous gel for fixing sandy soil, a preparation method of the green microbial calcareous gel, a green microbial calcareous gel The application in the consolidation of sand, the use method of the green microbial calcareous glue in the consolidation of sand, the sand column made by using the green microbial calcium glue, and the preparation method of the sand column. Background technique [0002] For the treatment of existing building foundations, the reinforcement of dam areas, the closure of permeable layers around mines, the repair of concrete cracks, and the repair of stone materials in ancient buildings, the most commonly used methods are cement slurry and chemical slurry. There have been studies to improve the chemical slurry to reduce the pollution of the original soil area after the application of the slurry. For example, the patent...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12N1/20C09K17/14E02D3/12C12R1/07
CPCC09K17/14C12N1/20C12P19/04E02D3/12
Inventor 李驰王燕星李弘岩王翠艳斯日古楞高瑜韩红伟
Owner INNER MONGOLIA UNIV OF TECH
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