A kind of cryopreservation liquid of leukemia cell sample and preparation method thereof

Abstract

The invention discloses cryopreservation fluid for leukemic cell samples, and a preparation method of the cryopreservation fluid, belonging to the technical field of cell cryopreservation. The cryopreservation fluid is prepared from a solution A, autologous plasma and dimethyl sulfoxide (DMSO), wherein the volume ratio of the solution A to the autologous plasma to the DMSO is equal to 1 to 1 to 0.04; the solution A is a voluven solution and is prepared from vitamin A, vitamin C, tea polyphenols and trehalose, wherein the final concentrations of the vitamin A, the vitamin C, the tea polyphenolsand the trehalose are respectively 10mg / mL, 10mg / mL, 0.5mg / mL and 20mg / mL. The cryopreservation fluid provided by the invention is obtained by evenly mixing the solution A, the autologous plasma andthe DMSO according to a ratio. The cryopreservation fluid is simple to prepare and low in cost, can be used for preserving leukemic cells for a long time, and enables the cell activity not to change;after the cryopreservation fluid is used, the survival rate of resurrected cells can reach 95 or above.

Description

technical field [0001] The invention relates to the technical field of cell cryopreservation, in particular to a cryopreservation solution for leukemia cell samples and a preparation method thereof. Background technique [0002] Leukemia is one of the most important tumors that endanger human beings. In the study of leukemia diagnosis and treatment, clinically frozen leukemia cell specimens play an important role in the diagnosis and classification of leukemia, the research of disease monitoring and guiding treatment, and the construction of clinical sample banks. Therefore, it is an urgent need in this field to develop a simpler and more effective method for cryopreserving leukemia specimens. Establishing a method for long-term cryopreservation of leukemia cells is of great significance for research on the diagnosis and treatment of leukemia. [0003] If it is simply frozen, the cells will be damaged in two aspects during the freezing process: First, during the freezing pro...

AI Technical Summary

Problems solved by technology

Using commercial plasma-free medium instead of animal plasma to culture cells still has some problems in practical application, such as high cost of medium development, lack of versatility, limited to specific cell culture, etc.

Adding specific cytokines to the medium is more expensive, and its extraction and preparation process is complicated, the yield is low, the cost is considerable, and it also has the disadvantages of potential toxicity and gene mutation, which make it unable to meet the needs of clinical applications.

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