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Long-term preservation method of rice blast strains

A technology for long-term preservation and rice blast fungus, which is applied in the field of long-term preservation of rice blast strains, can solve the problems of strain variation, small filter paper, easy pollution, etc., and achieves the effects of simple activation, less pollution, and less workload.

Inactive Publication Date: 2018-11-23
XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the test tube is small, and the amount of filter paper is small for each storage. One strain needs multiple test tube slopes for culture and preservation. The potato agar medium is rich in nutrients, easy to pollute, and prone to strain variation

Method used

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  • Long-term preservation method of rice blast strains
  • Long-term preservation method of rice blast strains
  • Long-term preservation method of rice blast strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The long-term preservation method of rice blast bacterial strain comprises the following steps:

[0034] 1) Preparation of corn flour rice straw agar medium (RCA): The medium was prepared according to a conventional method, and the medium components were: 40 g of corn flour, 20 g of agar, 20 g of rice straw powder, and 1000 ml of sterile water.

[0035] 2) Autoclave, pour into a culture dish to make a culture plate;

[0036] 3) spreading the filter paper sheet on the solidified corn meal rice straw agar medium plate;

[0037] 4) inoculating the rice blast bacterial strain after the activation of the potato agar medium (PDA) on the corn flour rice straw agar medium plate;

[0038] 5) Place the medium culture dish upside down, and cultivate it at 25°C until the culture dish is covered with strains;

[0039] 6) Take out the filter paper with tweezers and place it in a paper bag;

[0040] 7) Indicate the strain information and storage time on the paper bag;

[0041] 8) ...

Embodiment 2

[0044] The rice blast bacterial strain long-term preservation method comprises the following steps:

[0045] 1) Preparation of corn flour rice straw agar medium (RCA): The medium was prepared according to a conventional method, and the medium components were: 40 g of corn flour, 20 g of agar, 40 g of rice straw powder, and 1000 ml of sterile water.

[0046] 2) Autoclave, pour into a culture dish to make a culture plate;

[0047] 3) spreading the filter paper sheet on the solidified corn meal rice straw agar medium plate;

[0048] 4) inoculating the rice blast bacterial strain after the activation of the potato agar medium (PDA) on the corn flour rice straw agar medium plate;

[0049] 5) Place the medium culture dish upside down, and place it in a 28°C incubator until the culture dish is covered with strains;

[0050] 6) Take out the filter paper with tweezers and place it in a paper bag;

[0051] 7) Indicate the strain information and storage time on the paper bag;

[0052...

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Abstract

The invention discloses a long-term preservation method of rice blast strains. According to the method, filter paper serves as a preservation medium and long-term preservation of the rice blast strains is realized. The method comprises the following implementation steps: 1) preparing a corn flour straw agar culture medium; 2) placing a filter paper piece on a solidified corn flour straw agar culture medium flat plate; 3) inoculating the activated rice blast strains to the flat plate; 4) taking out the filter paper full of the rice blast strains; 5) performing drying treatment on the filter paper in a super-clean bench; and 6) preserving the filter paper at -20 DEG C. The method is simple, lower in pollution and long in preservation cycle, the activation times are reduced, and the characters of the strains are stable; meanwhile, the preservation space is saved and the strains are convenient to activate.

Description

technical field [0001] The invention belongs to the technical field of bacterial strain preservation, and relates to a method for long-term preservation of rice blast strains. Background technique [0002] Rice blast is a worldwide disease caused by ascomycete fungi, which can cause a substantial reduction in rice production, in severe cases, by 40% to 50%, or even failure. Rice blast can occur throughout the growth period of rice, and has the characteristics of long damage time, diverse symptoms and multiple infection sites. Due to the genetic complexity of the rice blast fungus race and the variability of pathogenicity, the resistance of resistant varieties will be weakened or lost after 3-5 years of popularization and planting. Breeding and planting rice blast-resistant varieties is the most economical, effective and safe measure to prevent and control rice blast. Real-time and accurate understanding and mastery of the population composition of rice blast fungi and the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/645
CPCC12N1/04
Inventor 何弯弯王健康丁成伟郭荣良吴玉玲徐家安王友霜胡婷婷赵轶鹏
Owner XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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