Identification method of manuka honey based on nuclear magnetic resonance technology

A manuka honey, nuclear magnetic resonance technology, which is applied in the analysis by nuclear magnetic resonance, material analysis by resonance, instruments, etc., can solve problems such as influence, and achieve short analysis time, strong controllability, and strong integrity. Effect

Active Publication Date: 2021-03-02
南京海关动植物与食品检测中心 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the current domestic research on manuka honey is basically blank, which will have a great impact on the supervision and supervision of imported manuka honey and the protection of the legitimate rights and interests of Chinese consumers

Method used

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  • Identification method of manuka honey based on nuclear magnetic resonance technology
  • Identification method of manuka honey based on nuclear magnetic resonance technology
  • Identification method of manuka honey based on nuclear magnetic resonance technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Determination of Characteristic Markers

[0033] 1. Instruments and equipment

[0034] Experimental equipment includes: Advance 400 nuclear magnetic resonance instrument (Bruker, Switzerland), 14.1 T superconducting magnet, 5 mm dual-core z-gradient probe and Topspin 2.3 test control and data processing software; 5 mm nuclear magnetic resonance sample tube; high-speed centrifuge (Sigma , German company); Vortex mixer (XW-80A, Shanghai Medical University Instrument Factory); LP403 analytical balance (Sartorius, German company);

[0035] The experimental solvents include: deuterated water (99.8% deuterium band) was purchased from Cambridge Isotope Laboratories; 3-trimethylsilyl-1-propyl sodium sulfonate (TSPSA) was purchased from Aldich-Sigma; Potassium and sodium dihydrogen phosphate (excellent grade) were purchased from Aldich-Sigma.

[0036] 2. Honey Sample Collection

[0037] A total of 7 varieties of manuka honey, kanuka honey, rivariwa honey, kamahi honey...

Embodiment 2

[0048] The samples submitted for inspection are respectively labeled as 1, 2, 3, and 4.

[0049] Pretreat the sample according to its nature, specifically:

[0050] For commercially available honey samples, stir them evenly, accurately weigh 0.25 g of samples into a centrifuge tube, add 1 mL of heavy water, and dissolve completely; take 0.8 μl of the above solution, add 100 μl of 0.5 mol / L phosphate buffer solution (pH =2.0), wherein the buffer solution contains 0.05% volume fraction of 3-trimethylsilyl-1-propyl sodium sulfonate internal standard solution and 0.1 mM sodium azide, vortexed to mix evenly, and then Use 1.0 mol / L hydrochloric acid and sodium hydroxide to adjust the pH of the final solution to 2.5, and take 600 μl of it in an NMR tube.

[0051] For the honey sample, stir it evenly, filter it with a nylon filter cloth with a pore size of 0.10mm-0.14mm, remove the solid impurities in the honey, accurately weigh 0.25 g of the sample in a centrifuge tube, add 1mL of h...

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Abstract

The invention belongs to the field of food safety, especially the field of manuka honey identification, and more specifically relates to a method for identifying manuka honey based on nuclear magnetic resonance technology. The method analyzes the nuclear magnetic resonance spectrum data of samples to be detected, Determine if the sample to be tested is manuka honey. The nuclear magnetic resonance-based Manuka honey identification method disclosed in the present invention can make up for the current defects in the identification of adulterated Manuka honey through specific characteristic markers. This method has the advantages of short analysis time, strong integrity, good specificity, strong controllability, and high accuracy. After using the identification method disclosed in the present invention, the minimum detection amount of the Manuka characteristic marker components contained in Manuka honey is 10%.

Description

technical field [0001] The invention belongs to the field of food safety, in particular to the field of manuka honey identification, and more specifically relates to a method for identifying manuka honey based on nuclear magnetic resonance technology. Background technique [0002] Manuka (Manuka) honey is a precious honey species unique to New Zealand. It is a shrub plant unique to New Zealand - Manuka ( Leptospermum scoparium ) brewed from nectar. Because Manuka honey has unique antibacterial activity, it can be used to prevent wound infection, help wound healing, and has high medicinal value, so it is more and more favored by Chinese consumers. [0003] At present, New Zealand's manuka honey exports have increased by about 10% every year for the past 10 years. In 2013, the value of honey exported from New Zealand was 128 million New Zealand dollars. According to statistics from a New Zealand beekeeper association, New Zealand only produces about 1,700-2,000 tons of Manuk...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N24/08
CPCG01N24/082
Inventor刘芸丁涛吴斌张建刘艳费晓庆陈磊陆慧媛黄娟
Owner南京海关动植物与食品检测中心