Primer, probe, kit and method for detecting streptococcus suis based on digital PCR technology

Abstract

The invention discloses a primer and probe, a kit and a method for detecting streptococcus suis based on the digital PCR technology. The primer and probe include a forward primer FP, a backward primerBP and a Probe. The detection kit includes 2 x ddPCR Supermix, ultrapure water without RNA enzymes, a bacterial total DNA extraction reagent, negative control and positive control. The detection method comprises the steps that DNA of a sample to be tested is extracted, the DNA of the sample to be tested is quantitatively detected by the microdrop digital PCR technology, a copy number obtained byamplification analysis is used for determining whether the sample to be tested contains the streptococcus suis or not, and the content of the streptococcus suis is determined. The primer and probe, the kit and the method for detecting the streptococcus suis based on the digital PCR technology have the advantages of accuracy, sensitivity, wide applicability, no dependence on a standard curve, realization of absolute quantification and the like, can realize early detection, early treatment and early prevention of the diagnosis of the streptococcus suis disease, and can effectively control the disease outbreak.

Description

technical field

[0001] The invention belongs to the technical field of molecular biology, and relates to a primer, a probe, a kit and a method for detecting Streptococcus suis based on digital PCR technology. Background technique

[0002] Streptococcus suis (SS) is a common opportunistic pathogen in pigs, which usually settles in the upper respiratory tract (especially the tonsils and nasal cavity), reproductive tract or digestive tract of pigs, and in severe cases, pigs will Symptoms such as meningitis, sepsis, pneumonia and arthritis occur, which can lead to sudden death in young pigs. Streptococcus suis can be divided into 35 serotypes (types 1-34 and type 1 / 2) according to the different characteristics of streptococcus capsular antigens, among which types 1, 2, 7, and 9 are pathogenic bacteria of pigs. And it can also cause human infection. Serious diseases such as meningitis, sepsis, pneumonia, arthritis, endocarditis, peritonitis and endophthalmitis will also occur af...

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