Schizochytrium sp. with high DHA content and application thereof

A technology of Schizochytrium content, applied in microorganism-based methods, microorganisms, fungi, etc., can solve the problems of high price, limited application of DHA, low overall yield of DHA, etc., and achieve the effect of fast growth and good economic benefits

Inactive Publication Date: 2018-12-07
ALGAEPRO BIOTECH CO LTD
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  • Description
  • Claims
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Problems solved by technology

However, the current microalgae industry mainly relies on autotrophic growth for open pond culture, and the

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  • Schizochytrium sp. with high DHA content and application thereof
  • Schizochytrium sp. with high DHA content and application thereof
  • Schizochytrium sp. with high DHA content and application thereof

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Embodiment Construction

[0023] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0024] 1. Acquisition and preservation of Schizochytrium sp. W1

[0025] The Schizochytrium of the present invention is obtained from water samples collected from East Lake in Wuhan City through artificial seawater screening and separation. The microalgae survives in the form of single cells, is spherical, has no flagella, does not move, and has a cell diameter of 4-30 μm. A colorless, transparent sphere capable of complete heterotrophic growth. After 18S rDNA sequencing and morphological identification, the microalgae belonged to the domain of eukaryotes, Chromalveolata, Heterokontophyta or Stramenopiles, Bigyra, Labyrinthulomycetes order, Thraustochytriaceae family, Schizochytridium ( Schizochytrium).

[0026] On November 13, 2017, the microalgae...

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Abstract

The present invention relates to Schizochytrium sp. with a high DHA content, application of the Schizochytrium sp. in preparation of DHA and a cultivation method of the Schizochytrium sp. The Schizochytrium sp. is preserved in China Center For Type Culture Collection on November 13, 2017, and the preservation number is CCTCCNO: M2017689. The Schizochytrium sp. W1 grows fast and can undergo complete heterotrophic growth. Under the condition of nitrogen deficiency induction, the Schizochytrium sp. can accumulate a large amount of the DHA. Therefore, the Schizochytrium sp. and the culture methodcan be used to prepare the DHA or food additives or feed additives with high DHA contents, and have good economic benefits.

Description

technical field [0001] The invention relates to the fields of microalgae cultivation and unsaturated fatty acid production, more particularly, a Schizochytrium sp. with high DHA content and application thereof. Background technique [0002] Polyunsaturated fatty acid (Polyunsaturated Fatty Acid, PUFA) refers to a straight-chain fatty acid containing two or more double bonds and a carbon length of 18-22 carbon atoms, mainly including linoleic acid (LA), γ-linolenic acid (GLA), α-linolenic acid (ALA), arachidonic acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), etc. In PUFA, the double bond farthest from the carboxyl terminal is called ω-3(n-3) series on the 3rd carbon atom, and ω-6(n-6) on the 6th carbon atom series. [0003] Docosahexaenoic acid (DHA) is the long-chain highly unsaturated fatty acid with the longest carbon chain and the highest degree of unsaturation found so far. DHA is an important structural component of biological membranes in the bo...

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Application Information

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IPC IPC(8): C12N1/14C12P7/64C12R1/645
CPCC12N1/14C12P7/6427C12N1/145C12R2001/645
Inventor 王强陈为先
Owner ALGAEPRO BIOTECH CO LTD
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