Detection method and detection kit for folic acid metabolism-related gene mutation loci

A folic acid metabolism and detection method technology, applied in the field of biomedicine, can solve the problems of lack of accurate and high-throughput gene mutation screening technology, hindering the research and development of precision medicine and clinical research, etc.

Inactive Publication Date: 2018-12-11
为康(苏州)基因科技有限公司
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Problems solved by technology

[0004] The lack of accurate and high-throughput gene mutation screening technology hinders the development and clinical research of precision medicine

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  • Detection method and detection kit for folic acid metabolism-related gene mutation loci
  • Detection method and detection kit for folic acid metabolism-related gene mutation loci
  • Detection method and detection kit for folic acid metabolism-related gene mutation loci

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Embodiment Construction

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0020] It should be noted that when a component is said to be "fixed" to another component, it can be directly on the other component or there can also be an intervening component. When a component is said to be "connected" to another component, it may be directly connected to the other component or there may be intervening components at the same time. When a component is said to be "set on" another component, it may be set directly on the other component or t...

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Abstract

The invention discloses a detection method for folic acid metabolism-related gene mutation loci. The detection method comprises the steps that a sample DNA is extracted; specific primers used for amplifying folic acid metabolism-related gene segments are designed; the single-tube multi-PCR amplification reaction is carried out so as to obtain target fragments; single-base extension primers used for the folic acid metabolism-related gene segments are designed; single base extension is carried out on the folic acid metabolism-related gene fragmentx; and nucleic acid mass spectrometry analysis iscarried out so as to measure the target DNA sequences. The detection method has the advantages that folic acid metabolism-related gene mutation screening can be effectively carried out on patients with colorectal cancer, the individual can be helped to fully understand the hereditary condition of the individual, and a clinical and scientific research foundation is laid for carrying out personalized accurate diagnosis and treatment on the patients. The invention further relates to a detection kit for the folic acid metabolism-related gene mutation loci.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a detection method and a detection kit for folic acid metabolism-related gene mutation sites. Background technique [0002] The three most important genes involved in folate metabolism are methylenetetrahydrofolate reductase gene (MTHFR), methionine synthase reductase gene (MTRR) and methionine synthase (MTR). The study found that the activity of MTHFR is related to the C677T and A1298C gene loci; the activity of MTRR is related to the A66G gene locus; the activity of MTR is related to the A2756G gene locus. When the gene locus is mutated, the activity of MTHFR, MTRR and MTR will decrease, which will lead to insufficient folic acid utilization capacity. [0003] There is a certain proportion of risk genes in the Chinese population - 78% of Chinese people have gene mutations related to folic acid utilization ability, which leads to different people's ability to utilize folic acid, so f...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12Q1/6883
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2533/101
Inventor 王文忠田军龙胡军陈苏平卜云璇
Owner 为康(苏州)基因科技有限公司
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