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A combined quantitative detection test paper for inflammatory markers and its preparation method

A technology for quantitative detection and detection test paper, which is applied in the field of biomedicine and can solve problems such as low sensitivity

Active Publication Date: 2022-03-01
广州华澳生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above problems and facilitate the detection of human serum amyloid A / hypersensitive C-reactive protein / procalcitonin, the present invention provides a simple, reliable, low-cost and high-sensitivity inflammatory marker Combined with quantitative detection test paper; this detection method uses a magnetic field to concentrate the tested substance in the sample, and then uses low-background, high-sensitivity fluorescence immunochromatography to detect the tested substance, which solves the problem of low sensitivity of POCT products

Method used

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  • A combined quantitative detection test paper for inflammatory markers and its preparation method
  • A combined quantitative detection test paper for inflammatory markers and its preparation method
  • A combined quantitative detection test paper for inflammatory markers and its preparation method

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Experimental program
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Embodiment

[0037] Embodiment, the establishment of detection method:

[0038] 1.1 Preparation of polystyrene microsphere seeds

[0039] 1.1.1 Measure 100ml of styrene and put it in a separatory funnel, wash it repeatedly with 1 M NaOH 3 times to remove the impurities in styrene, then wash it with purified water until neutral, then put it in a drying dish to dry for more than 24 hours;

[0040] 1.1.2 Fill the reaction device with argon gas to remove the oxygen in the device;

[0041] 1.1.3 Add 100ml of ethanol to the reaction device, then add 5g of PVP, and stir for 30min;

[0042] 1.1.4 Add 40ml of washed styrene and 5ml of azobisisobutyronitrile (AIBN), raise the temperature of the water bath to 45°C, react for 30min, then raise the reaction temperature to 75°C, and react for 12h;

[0043] 1.1.5 After the reaction is over, filter the above reaction solution with a mesh sieve, then wash and centrifuge with absolute ethanol for 3 to 5 times, then put the polymer after the initial impuri...

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Abstract

A combined quantitative detection test strip for inflammatory markers, the inflammatory markers are serum amyloid A, hypersensitive C-reactive protein and procalcitonin, the test strip includes sample pads, inflammatory marker antibody magnetic rare earth fluorescent microspheres There are three quantitative detection lines of inflammatory markers and a C line in the quality control area on the coating film. After the test substance in the sample is concentrated by a magnetic field, a fluorescent immune layer with low background and high sensitivity is used to Analytical method to detect the substance to be tested, which solves the problem of low sensitivity of POCT products. It can avoid the interference of other substances in the blood when the immunological lateral chromatography reagent detects the blood sample.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a combined quantitative detection test paper for inflammatory markers based on magnetic rare earth fluorescent microspheres as a tracer marker and a preparation method thereof, specifically, a method for measuring blood (serum, plasma, whole blood) Reagent card for the detection of serum amyloid A / high-sensitivity C-reactive protein / procalcitonin content. Background technique [0002] Serum Amyloid A Protein (SAA) is an acute phase reaction protein mainly secreted by liver cells, belonging to the heterogeneous protein in the apolipoprotein family, with a relative molecular weight of about 12,000. According to the expression in vivo, SAA can be divided into acute SAA (acute SAA, A-SAA) and constitutive SAA (constitutive SAA, C-SAA). SAA in normal human body mainly comes from C-SAA expressed constitutively in liver cells. When the body is stimulated by infection, inflammation, injury, etc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/533
CPCG01N33/533G01N33/6893
Inventor 于龙波于永涛黎权刘日俊
Owner 广州华澳生物科技有限公司