Cell nucleus segmentation method and system for fluorescence in situ hybridization images

A technology of fluorescence in situ hybridization and cell nucleus, which is applied in the field of cell nucleus segmentation method and system of fluorescence in situ hybridization image, which can solve the problems of cell adhesion and blurred cell nucleus edge, so as to improve over-segmentation, improve segmentation quality, and reduce image fluorescence The effect of the scattering effect

A technology of fluorescence in situ hybridization and cell nucleus, which is applied in the field of cell nucleus segmentation method and system of fluorescence in situ hybridization image, which can solve the problems of cell adhesion and blurred cell nucleus edge, so as to improve over-segmentation, improve segmentation quality, and reduce image fluorescence The effect of the scattering effect

CN109003255AActive Publication Date: 2018-12-14武汉海星通技术股份有限公司
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  • Cell nucleus segmentation method and system for fluorescence in situ hybridization images
  • Cell nucleus segmentation method and system for fluorescence in situ hybridization images
  • Cell nucleus segmentation method and system for fluorescence in situ hybridization images

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Embodiment Construction

[0041] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, but these embodiments should not be construed as limiting the present invention.

[0042] The present invention proposes a flow process of a cell nucleus segmentation method for fluorescence in situ hybridization images as follows: figure 1 Shown, is for a given FISH image of breast cancer (such as figure 2 shown) to find the process of the nucleus region, the specific steps include:

[0043] 1) The preprocessed FISH image (such as image 3As shown), the pixel value of each pixel of the red, green, and blue three-channel images in the image is reduced by adt_value to ensure that any position of the image is still greater than or equal to 0 after the reduction, and the overall displacement of the image is realized; the value range of adt_value is [2050 ] pixels. Collect the red channel and green channel in the three-channel image where the pi...

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Abstract

The invention discloses a cell nucleus segmentation method and system of fluorescence in situ hybridization images. The method realizes the recognition and segmentation of cell nucleus in FISH imagesthrough pretreatment, binarization, hole filling, morphological operation, secondary watershed and other processing processes. The system comprises a FISH image receiving module, an image preprocessing module, a cell nucleus detecting module and a report generating module. When the FISH images of breast cancer, gastric cancer and other cancer cells are segmented to obtain the cell nucleus region,the invention can reduce the image fluorescence scattering effect through multiple pretreatments, can effectively improve the over-segmentation problem easily caused by the direct watershed, and thusimprove the segmentation quality of the FISH image recognition cell.

Description

technical field [0001] The invention relates to the technical field of medical pathology analysis, which uses a computer image processing method to automatically identify cell nucleus regions in fluorescence in situ hybridization images, and more specifically refers to a method and system for segmenting cell nuclei in fluorescence in situ hybridization images. Background technique [0002] In the field of cancer treatment, such as breast cancer and gastric cancer, the targeted drug Herceptin has the characteristics of high efficiency, low toxicity and strong selectivity. It is a human source of high-purity recombinant DNA derivatives targeting Her-2 / neu receptors Monoclonal Antibodies. Since the targeted drug Herceptin is only effective for breast cancer with HER2 amplification, accurate detection of HER2 amplification is an absolute prerequisite for clinical application of Herceptin, and is also the premise and key to successful targeted therapy. For clinical routine and r...

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Application Information

Patent Timeline
14 Dec 2018
Publication
CN109003255A
IPC
G06T7/00; G06T7/11; G06T5/00; G06T7/90
CPC
G06T7/0012; G06T7/11; G06T7/90; G06T2207/30024; G06T5/92; G06T5/80; G06T5/70
Inventors
刘娟; 陈哲