Cell nucleus segmentation method and system for fluorescence in situ hybridization images

Abstract

The invention discloses a cell nucleus segmentation method and system for fluorescence in situ hybridization images. The method realizes the identification of cell nuclei in FISH images through preprocessing, binarization, hole filling, morphological operations, secondary watershed and other processing processes. ,segmentation. The system includes a FISH image receiving module, an image preprocessing module, a cell nucleus detection module and a report generating module. When segmenting FISH images of breast cancer, gastric cancer and other cancer cells to obtain the cell nucleus area, the present invention can reduce the image fluorescence scattering effect through multiple preprocessing, which can effectively improve the problem of over-segmentation in direct watersheds, thereby improving FISH Segmentation quality of image recognition cells.

Description

technical field

[0001] The invention relates to the technical field of medical pathology analysis, which uses a computer image processing method to automatically identify cell nucleus regions in fluorescence in situ hybridization images, and more specifically refers to a method and system for segmenting cell nuclei in fluorescence in situ hybridization images. Background technique

[0002] In the field of cancer treatment, such as breast cancer and gastric cancer, the targeted drug Herceptin has the characteristics of high efficiency, low toxicity and strong selectivity. It is a human source of high-purity recombinant DNA derivatives targeting Her-2 / neu receptors Monoclonal Antibodies. Since the targeted drug Herceptin is only effective for breast cancer with HER2 amplification, accurate detection of HER2 amplification is an absolute prerequisite for clinical application of Herceptin, and is also the premise and key to successful targeted therapy. For clinical routine and r...

Images