Synbiotic oral spray preparation for hatched young meat chickens and preparation method thereof
An oral spray and synbiotic technology, which can be used in applications, additional food elements, animal feed, etc., can solve the problems of healthy intestinal development and low feed intake, and can enhance immune function, improve digestibility, and preparation methods. simple effect
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Embodiment 1
[0020] Inoculate Lactobacillus plantarum, Lactobacillus bulgaricus, Bacillus subtilis, Bacillus licheniformis, Bifidobacterium, Enterococcus faecalis and yeast into their respective appropriate medium, culture at 37.5°C for 36 hours, take 150mL of seed culture and inoculate The fermenter continued to expand and cultivate until the concentration of viable bacteria reached 3×10 8 When CFU / mL, stop the culture. The fermentation broth was centrifuged at 4500rpm for 20min at 8°C, and the cells in the lower layer were collected and mixed by equal weight. Take 150 parts of the cell mixture, 100 parts of sodium alginate, 50 parts of attapulgite and 50 parts of dextrin, and stir at 100 rpm for 10 minutes at 8°C to fully absorb the cells. Spray 250 parts of 7% skim milk into the mixture, stir at 100 rpm at 8°C for 10 minutes, and then dry at low temperature for 3 hours at 8°C, then pulverize to 100 mesh. Take 600 parts of mixed probiotic preparations, 200 parts of manno-oligosaccharid...
Embodiment 2
[0022] Inoculate Lactobacillus salivarius, Lactobacillus bulgaricus, Bacillus subtilis, Bacillus laniciformis, Bifidobacterium, Enterococcus faecalis and yeast into their respective appropriate medium, culture at 37.5°C for 36h, take 150mL of seed culture to inoculate into the fermenter to continue to expand the culture until the concentration of viable bacteria reached 3 × 10 8 When CFU / mL, stop the culture. The fermentation broth was centrifuged at 4500rpm for 20min at 8°C, and the cells in the lower layer were collected and mixed by equal weight. Take 150 parts of the cell mixture, 100 parts of sodium alginate, 50 parts of zeolite powder and 50 parts of dextrin, and stir at 100 rpm for 10 minutes at 8°C to fully absorb the cells. Spray 250 parts of 7% skim milk into the mixture, stir at 100 rpm at 8°C for 10 minutes, and then dry at low temperature for 3 hours at 8°C, then pulverize to 100 mesh. Take 600 parts of mixed probiotic preparations, 200 parts of lactose oligosac...
Embodiment 3
[0024] Inoculate Lactobacillus plantarum, Lactobacillus bulgaricus, Bacillus subtilis, Bacillus laniciformis, Bacillus licheniformis, Bifidobacterium, Enterococcus faecalis and yeast into their respective appropriate medium, culture at 37.5°C for 36 hours, and take the seeds 150mL of the culture was inserted into the fermenter to continue to expand the culture until the concentration of viable bacteria reached 3×10 8 When CFU / mL, stop the culture. The fermentation broth was centrifuged at 4500rpm for 20min at 8°C, and the cells in the lower layer were collected and mixed by equal weight. Take 150 parts of the cell mixture, 100 parts of sodium alginate, 50 parts of attapulgite and 50 parts of dextrin, and stir at 100 rpm for 10 minutes at 8°C to fully absorb the cells. Spray 250 parts of 7% skim milk into the mixture, stir at 100 rpm at 8°C for 10 minutes, and then dry at low temperature for 3 hours at 8°C, then pulverize to 100 mesh. Take 600 parts of mixed probiotic prepara...
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