Method for extracting chlorogenic acid from tobacco waste by combining high-voltage pulse electric field, microwaves and ultrasonic waves

A high-voltage pulsed electric field and ultrasonic technology, applied in the separation/purification of carboxylic acid esters, organic chemistry, etc., to achieve high extraction rate, short extraction time, and simple operation

Active Publication Date: 2019-01-01
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Polyphenols such as chlorogenic acid are ubiquitous in plants. Traditional techniques are mostly extracted from honeysuckle and Eucommia leaves, and extraction from tobacco waste is rarely reported. High-voltage pulsed electric field combined with microwave and ultrasonic waves are used to extract green leaves from tobacco waste. Ortho acid has not been reported

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  • Method for extracting chlorogenic acid from tobacco waste by combining high-voltage pulse electric field, microwaves and ultrasonic waves

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Embodiment 1

[0026] A method for extracting chlorogenic acid in tobacco waste in combination with high-voltage pulsed electric field microwaves and ultrasonic waves, comprising the following steps:

[0027] (1) 100g of tobacco waste is dried at 50°C, pulverized, and passed through a 20-mesh sieve;

[0028] (2) Put the pulverized tobacco waste in a solvent composed of 560mL of acetone and 560mL of water, and add 12g of branched 13-carbon Guerbet alcohol polyoxyethylene ether, stir well and let it stand for 1h. 200W, 10GHz microwave treatment twice, 30s each time, and then 1ms under the high-voltage pulsed electric field with a field strength of 25kV / cm and a pulse frequency of 400pps, and then use a hydrochloric acid solution with a molar concentration of 0.1mol / L Adjust the pH to 5, extract for 30 minutes under ultrasonic waves with a power of 300W and a frequency of 30KHz, filter with suction, collect the filtrate, repeat the above steps to extract the filter residue once more, filter wit...

Embodiment 2

[0032] A method for extracting chlorogenic acid in tobacco waste in combination with high-voltage pulsed electric field microwaves and ultrasonic waves, comprising the following steps:

[0033] (1) 100g of tobacco waste is dried at 55°C, pulverized, and passed through a 30-mesh sieve;

[0034] (2) Put the pulverized tobacco waste in a solvent composed of 524mL of acetone and 786mL of water, and add 24g of 12-14 carbon primary alcohol polyoxyethylene ether, stir well and let it stand for 1.5h. Treat 3 times under 15GHz microwave, 25s each time, and then treat 2ms under high-voltage pulsed electric field with field strength of 32kV / cm and pulse frequency of 500pps, and then adjust pH to 5.5 with hydrochloric acid solution with molar concentration of 0.1mol / L , extract 25min under ultrasonic waves with a power of 350W and a frequency of 35KHz, suction filtration, and collect the filtrate. Refrigerate in a refrigerator at 3°C ​​for 36 hours, remove the bottom precipitate, recover...

Embodiment 3

[0038] A method for extracting chlorogenic acid in tobacco waste in combination with high-voltage pulsed electric field microwaves and ultrasonic waves, comprising the following steps:

[0039] (1) drying the tobacco waste at 60°C, pulverizing, and passing through a 40-mesh sieve;

[0040] (2) Place the pulverized tobacco waste in a solvent mixed with 538mL acetone and 1076mL water, add 42g fatty acid methyl ester polyoxyethylene ether, stir well and let it stand for 2h, at a power of 300W, a frequency of Treat 3 times under 20GHz microwave, 20s each time, then treat 1ms under the high-voltage pulsed electric field with field strength 40kV / cm and pulse frequency 600pps, and then adjust the pH to 6 with a hydrochloric acid solution with a molar concentration of 0.1mol / L. Power is 400W, frequency is 40KHz ultrasonic extraction 20min, suction filtration, collect filtrate, filter residue repeat above-mentioned steps to extract again, suction filtration, merge twice filtrate, aband...

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Abstract

The invention discloses a method for extracting chlorogenic acid from tobacco waste by combining a high-voltage pulse electric field, microwaves and ultrasonic waves. The method comprises: (1) dryingtobacco waste, crushing, and screening; (2) placing the crushed tobacco waste in a solvent, standing, carrying out microwave treatment, treating with a high-voltage pulse electric field, adjusting thepH value with a hydrochloric acid solution, carrying out ultrasonic wave treatment, carrying out suction filtering, collecting the filtrate, extracting the filtering residue by repeatedly performingthe previous steps, carrying out suction filtering, combining the two filtrates, removing the filtering residue, adding a protection agent to the filtrate, refrigerating in a refrigerator, removing the bottom layer precipitate, carrying out rotary evaporation to recover acetone, concentrating, carrying out centrifugal separation, and taking the supernatant; (3) carrying out wet column loading on amacroporous adsorption resin, adding the supernatant, adsorbing, carrying out elution with an ethanol aqueous solution after the adsorbing, and collecting the eluent; and (4) carrying out rotary evaporation on the prepared eluent to remove the ethanol, concentrating, placing into the refrigerator, freezing, placing into a freeze dryer, and drying to obtain the crude chlorogenic acid. With the method of the present invention, the chlorogenic acid extraction rate is high.

Description

technical field [0001] The invention relates to a method for extracting chlorogenic acid, in particular to a method for extracting chlorogenic acid in tobacco waste by combining high-voltage pulse electric field with microwave and ultrasonic waves. Background technique [0002] Chlorogenis Acid, also known as coffee tannin, is a phenylpropanoid compound synthesized by plants during aerobic respiration. Modern pharmacological research shows that chlorogenic acid is an important physiologically active substance and has the reputation of "plant gold". It not only has antibacterial, antiviral, liver and gallbladder protection, lowering blood pressure, lowering blood fat, scavenging free radicals and stimulating the central nervous system and other functions, but also has a significant inhibitory effect on colorectal cancer, liver cancer and laryngeal cancer. Effective protectant against cancer. Studies suggest that chlorogenic acid will hopefully become a leading drug against ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C69/732
CPCC07C67/48C07C69/732
Inventor 卢燕回陈泽鹏
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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