Breeding method of super long-grain rice

A technology of super-long rice, applied in the direction of chemical instruments and methods, biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effects of saving production costs, shortening the breeding cycle, and improving selection efficiency

Active Publication Date: 2021-07-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many cloned grain shape genes, there are few combinations available to improve the appearance quality

Method used

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  • Breeding method of super long-grain rice
  • Breeding method of super long-grain rice
  • Breeding method of super long-grain rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Location of grain length main gene GL3.3

[0061] 1. Construction of recombinant inbred line population

[0062] F 1 (parental seed morphology see figure 2 Figure A in ), and then self-crossed, a total of 190 strains of F 2 group. The F of these 190 individual plants 2 The population can follow the "one-grain pass" method (Fang Xuanjun et al., 2000), and continue to self-cross until F 7 generation( figure 1 ).

[0063] 2. Genotype identification and genetic map construction

[0064]The identification method of genotype is conventional SSR analysis method. For specific steps, the first choice is to screen polymorphic SSR primers. A total of 574 pairs of SSR primers were screened in the present invention, including 452 pairs of primers of RM series and 118 pairs of primers of MRG series. RM series primers had 185 pairs of polymorphic markers between rice varieties Nanyangzhan and Zhenshan 97, and MRG series primers had 54 pairs of polymorphic markers...

Embodiment 2

[0069] Example 2: Functional verification and effect analysis of GL3.3

[0070] 1. Function verification of GL3.3

[0071] Os03g0841800 encodes a 6281kb open reading frame (ORF) consisting of 12 exons and 11 introns, annotated as GSK5 in the japonica rice "Nipponbare" reference genome (Kawahara et al., 2013). In order to further confirm whether Os03g0841800 is GL3.3, the applicant knocked out the GSK5 gene in the short-grain indica rice Zhenshan 97 using the CRISPR / Cas9 system (Shan et al., 2013). The applicant has obtained two kinds of mutants ( image 3 Figure A in the to image 3 Figure E in). Both mutant plants had significantly increased grain length and grain weight compared to wild-type ZS97 (non-transgenic) (see image 3 Figures C to E in image 3 in the figure). On the other hand, the applicant complementarily transformed the allelic gene of GL3.3 in "ZS97" in "NYZ" (Hiei et al., 1994). The results of the analysis showed that the transgenic negative plants had ...

Embodiment 3

[0078] Example 3: Application of GL3.3 in improving rice grain length

[0079] GL3.3 located in the present invention is a rare and major grain length gene, and can interact with GS3 to exert a stronger function to form a super-long grain phenotype, which can effectively improve the grain length of existing cultivated rice varieties traits. In the breeding process, molecular markers of GL3.3 can be used for assisted selection, which can reduce the planting area, simplify the breeding process and save manpower and material resources, so as to improve the breeding efficiency. Therefore, the rice germplasm material (such as the variety "Nanyangzhan" in this experiment) has the non-functional allele of GL3.3 (gl3.3 Aus and gl3.3 Jap ) materials are used to improve rice grain length traits, and have a very broad application prospect.

[0080] The technical route of this embodiment can be found in Figure 7 the method described, Figure 7It is the overall technical process desi...

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Abstract

The invention belongs to the technical field of rice molecular breeding. In particular, it relates to a method for cultivating super-long-grain rice. The present invention utilizes the method of map-based cloning to separate and clone a new rice grain length gene GL3.3 (the amino acid sequence encoded by the gene is shown as 1-1275bp in SEQ ID NO: 1), and the gene is consistent with the known grain length gene GL3.3. Polymerization of the gene GS3 (the amino acid sequence encoded by the gene is shown as 1‑696bp in SEQ ID NO: 3) can form super-long-grained rice materials. The KASP molecular marker primers of GS3 and GL3.3 can effectively select and aggregate the dominant alleles of the two in breeding, and the selection of super long-grain families with gs3gl3.3 genotype combination can significantly improve the quality of rice breeding efficiency.

Description

technical field [0001] The invention belongs to the technical field of rice molecular breeding. In particular, it relates to a method for cultivating super-long-grain rice. The present invention isolates and clones a new rice grain length gene GL3.3 by means of a map-based cloning method, and the gene can be aggregated with the known grain length gene GS3 to form super-long grain length rice materials. The KASP molecular marker primers of GS3 and GL3.3 can effectively select and aggregate the dominant alleles of the two in breeding, and the selection of super long-grain families with gs3gl3.3 genotype combination can significantly improve the quality of rice breeding efficiency. Background technique [0002] Rice is the main food crop in my country, and the appearance quality of rice is an important indicator to determine the commodity value of rice. For a long time, rice breeders and seed companies in my country have always regarded yield as the ultimate goal of breeding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415
CPCC07K14/415C12N15/8267C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 何予卿夏朵周浩张庆路高冠军
Owner HUAZHONG AGRI UNIV
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