Application of protein OsGATA6 in regulating and controlling flowering time, spike type, grain shape and thousand grain weight of plant
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A technology of flowering time and protein, applied in angiosperms/flowering plants, botanical equipment and methods, applications, etc.
Pending Publication Date: 2022-07-29
SHANGHAI JIAO TONG UNIV
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Although there have been many reports on the regulation mechanism of rice...
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Embodiment 1
[0084] Example 1. Acquisition of OsGATA6 gene
[0085] The inventors of the present invention have cloned a new GATA transcription factor OsGATA6 gene (LOC_Os01g54210) from rice after extensive experiments. Sequence analysis showed that the OsGATA6 gene contained two exons of 291 bp and 873 bp in size, encoding a protein composed of 387 amino acids.
[0086] The full-length nucleotide sequence of the OsGATA6 gene is shown in SEQ ID NO.1.
[0087] The nucleotide sequence of the coding region of the OsGATA6 gene is shown in SEQ ID NO.2.
[0088]The protein encoded by the OsGATA6 gene is named as protein OsGATA6, and its amino acid sequence is shown in SEQ ID NO.3.
Embodiment 2
[0089] Example 2. Application of protein OsGATA6 in regulating rice flowering time, panicle shape, grain shape and thousand-grain weight 1. Construction of recombinant plasmid
[0090] 1. Construction of recombinant plasmid pUN1301-OsGATA6
[0091] (1) Using the genomic DNA of rice cultivar ZH11 as a template, a primer pair consisting of OsGATA6OX-F and OsGATA6OX-R was used to perform PCR amplification to obtain PCR amplification products.
[0092] (2) The PCR amplification product was double digested with restriction enzymes BamHI and KpnI, and the digested product was recovered.
[0093] (3) The vector pUN1301 (Li D, Wang L, Wang M, XuYY, Luo W, Liu YJ, Xu ZH, Li J, Chong K (2009). Engineering OsBAK1 gene was digested with restriction enzymes BamHI and KpnI. As amolecular tool to improve rice architecture for high yield. Plant Biotechnol J. 7(8):791-806.), the vector backbone was recovered.
[0094] (4) Connect the enzyme digestion product recovered in step (2) and the vec...
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Abstract
The invention discloses application of a protein OsGATA6 in regulation and control of flowering time, spike type, grain shape and thousand grain weight of plants. The amino acid sequence of the protein OsGATA6 is shown as SEQ ID NO: 3. Experiments prove that the OsGATA6 gene is over-expressed in rice to obtain transgenic rice; the flowering time of the transgenic rice is delayed, the panicle shape is compact, the thousand seed weight is reduced, the grain length is reduced, and the grain width is reduced; the OsGATA6 gene is inhibited in rice, and rice with the OsGATA6 gene knocked out or rice with the OsGATA6 gene interfered is obtained. The flowering time of the OsGATA6 gene knockout rice and the OsGATA6 gene interference rice is advanced, panicle types are sparse, the thousand seed weight is increased, the grain length is increased, and the grain width is increased. Therefore, the protein OsGATA6 can regulate and control the flowering time, the ear type, the thousand seed weight, the grain length and the grain width of the plant. The method has an important application value.
Description
technical field [0001] The invention belongs to the field of biotechnology, in particular to the application of protein OsGATA6 in regulating plant flowering time, ear shape, grain shape and thousand-grain weight. Background technique [0002] Rice (Oryza sativa L.) is the main food crop in my country. Increasing the yield per unit area of rice has always been an important goal of breeding and production. Rice yield is determined by three factors: effective panicle number per unit area, grain number per panicle and grain weight. Effective panicle number is closely related to plant type and tiller. The number of grains per panicle is determined by the type of panicle, and the number of stems and spikelets produced by the development of the panicle (inflorescence) meristem determines the maximum number of grains per panicle. Increasing the number of grains per ear can significantly increase the yield without significantly affecting the seed setting rate. It has been repo...
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