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Pan-ELR<+> CXC chemokine antibodies

An antibody, chemotactic protein technology, applied in the direction of antibodies, anti-cytokine/lymphokine/interferon immunoglobulin, anti-tumor drugs, etc. 2 Combination and other issues

Active Publication Date: 2019-01-04
ELI LILLY & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the publication does not demonstrate that the antibody binds tightly to GCP-2 (CXCL6) and does not mention binding to NAP-2 (CXCL7)
[0006] However, no antibodies capable of binding and neutralizing all 7 human ELR+ CXC chemokines have been published

Method used

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  • Pan-ELR&lt;+&gt; CXC chemokine antibodies
  • Pan-ELR&lt;+&gt; CXC chemokine antibodies
  • Pan-ELR&lt;+&gt; CXC chemokine antibodies

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Embodiment

[0040] Expression and production of antibodies

[0041] The antibody used in the present invention can be expressed and purified as follows. NS0 cells were transfected with an expression vector comprising the DNA sequence of SEQ ID NO: 5 (encoding the heavy chain polypeptide of SEQ ID NO: 1) and SEQ ID NO: 6 (encoding the light chain polypeptide of SEQ ID NO: 3). The antibody obtained by this expression vector is "Antibody 1".

[0042] In addition, an expression vector containing the DNA sequence of SEQ ID NO: 17 (encoding the heavy chain polypeptide of SEQ ID NO: 13) and SEQ ID NO: 18 (encoding the light chain polypeptide of SEQ ID NO: 15) was used to transfect NS0 cells. The antibody obtained by this expression vector is "Antibody 2".

[0043] For antibody 1 or antibody 2, the transfection library is plated at low density to allow the growth of stable expression cells close to monoclonal. The main wells are screened for antibody expression and then scaled up in serum-free suspen...

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Abstract

Antibodies are provided that specifically bind seven human ELR<+> CXC chemokines. The antibodies of the invention are useful for treating various inflammatory / autoimmune diseases, such as inflammatorybowel disease (IBD), plaque psoriasis, and palmoplantar pustulosis; and cancer, such as renal cancer or ovarian cancer.

Description

[0001] This application is a Chinese patent application 201480013248.X "Pan-ELR" with the filing date of March 5, 2014 + Divisional application of "CXC Chemokine Antibody". Technical field [0002] The present invention relates to antibodies against ELR+CXC chemokines and their use in the treatment of diseases mediated by ELR+CXC chemokines. Background technique [0003] ELR+ CXC chemokines (so called because members of the chemokine family have ELR amino acid motifs next to their CXC motifs) play an important role in various pathogenesis, including neutrophils to inflammation and blood vessels Generate site migration. Neutrophils contribute to the onset of several acute and chronic inflammatory / autoimmune diseases, such as inflammatory bowel disease (IBD), psoriasis, and palmoplantar pustulosis. ELR+ CXC chemokines also play a key role in tumor formation and tumor metastasis. These chemokines are highly expressed in tumors. In the tumor environment, ELR+CXC chemokines are inv...

Claims

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Application Information

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IPC IPC(8): C07K16/24A61K39/395A61P17/06A61P17/00
CPCC07K16/24A61K2039/505C07K2317/34C07K2317/76C07K2317/33C07K2317/92A61P1/00A61P17/00A61P17/06A61P35/00
Inventor C.B.贝德勒K.K.基克利B.A.斯特里夫勒D.R.维彻尔J.S.博伊尔斯
Owner ELI LILLY & CO
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