Preparation method and application of a photoelectrochemical immunosensor based on tin dioxide/cadmium carbonate/cadmium sulfide
An immunosensor, tin dioxide technology, applied in scientific instruments, instruments, material analysis by electromagnetic means, etc., can solve problems such as unreported
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Embodiment 1
[0032] Example 1 A preparation method of a photoelectrochemical immunosensor based on tin dioxide / cadmium carbonate / cadmium sulfide:
[0033] 1) The ITO conductive glass was ultrasonically cleaned with detergent, acetone, ethanol, 0.1 mol / L sodium hydroxide solution and ultrapure water for 0.5 h, and dried under nitrogen;
[0034] 2) Take 6 µL of tin dioxide / cadmium carbonate / cadmium sulfide solution and drop it on the conductive surface of ITO conductive glass, and let it dry at room temperature;
[0035] 3) Soak the above-modified electrode in thioglycolic acid solution with a concentration of 8 mmol / L for 0.5 h, and then wash it with ultrapure water;
[0036] 4) Drop 400 mmoL / L 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride solution and 200 mmoL / L N-hydroxy Mix 4 μL of succinimide solution. After incubating at room temperature for 0.5h, rinse the electrode surface with ultrapure water, and dry it to a wet film state at room temperature;
[0037] 5) Add dropwise...
Embodiment 2
[0040] Example 2 A preparation method of a photoelectrochemical immunosensor based on tin dioxide / cadmium carbonate / cadmium sulfide:
[0041] 1) The ITO conductive glass was ultrasonically cleaned with detergent, acetone, ethanol, 0.1 mol / L sodium hydroxide solution and ultrapure water for 0.5 h, and dried under nitrogen;
[0042] 2) Take 6 µL of tin dioxide / cadmium carbonate / cadmium sulfide solution and drop it on the conductive surface of ITO conductive glass, and let it dry at room temperature;
[0043] 3) Soak the above-modified electrode in a thioglycolic acid solution with a concentration of 3 mmol / L for 0.5 h, and then wash it with ultrapure water;
[0044] 4) Drop 10 mmol / L 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride solution and 20 mmol / L N-hydroxy Mix 4 μL of succinimide solution. After incubating at room temperature for 0.5h, rinse the electrode surface with ultrapure water, and dry it to a wet film state at room temperature;
[0045] 5) Add dropwi...
Embodiment 3
[0048] Embodiment 3 The preparation of tin dioxide / cadmium carbonate / cadmium sulfide solution:
[0049] Add 0.64 g of tin tetrachloride pentahydrate, 0.54 g of cadmium dichloride and 0.37 g of thiourea to 30 mL of ultrapure water in sequence and stir magnetically for 30 min; then, add ammonia solution with a concentration of 10.5 mol / L dropwise The pH value of the solution reached 7.5; the above solution was stirred for 10 min and then transferred to the reaction kettle, and reacted at 140 °C for 16 h; naturally cooled; the obtained product was washed with absolute ethanol and ultrapure water for 3 times; vacuum-dried at 40 °C for 12 h. h; Tin dioxide / cadmium carbonate / cadmium sulfide material was obtained after grinding; and configured into a tin dioxide / cadmium carbonate / cadmium sulfide aqueous solution with a concentration of 1 mg / mL.
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