Method for promoting the production of N-acetylneuraminic acid by fermentation of Bacillus subtilis

A technology of acetylneuraminic acid and Bacillus subtilis, which is applied in the field of Bacillus subtilis fermentation to produce N-acetylneuraminic acid, can solve the problems of difficult to meet the needs of use and low fermentation yield, and achieve the effect of increasing yield

Active Publication Date: 2020-08-07
武汉中科光谷绿色生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, using the constructed Bacillus engineering bacteria to produce food, pharmaceuticals and other additive raw materials and materials, such as N-acetylneuraminic acid, has high safety and great application prospects, but Bacillus genes such as Bacillus subtilis The fermentation yield of N-acetylneuraminic acid produced by engineering bacteria is low, and it is difficult to meet the needs of use

Method used

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  • Method for promoting the production of N-acetylneuraminic acid by fermentation of Bacillus subtilis
  • Method for promoting the production of N-acetylneuraminic acid by fermentation of Bacillus subtilis
  • Method for promoting the production of N-acetylneuraminic acid by fermentation of Bacillus subtilis

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Embodiment 1

[0032] The embodiment of the present invention provides a method of using Schizochytrium to promote the fermentation of Bacillus subtilis to produce N-acetylneuraminic acid, which mainly includes the following steps:

[0033]S11. Cultivate Schizochytrium, take out the Schizochytrium strains preserved in glycerol tubes from the -80°C freezer, and transfer 0.3mL of Schizochytrium strains to the In the 250mL Erlenmeyer flask of 50mL liquid seed culture medium, cultivate 48h under the rotating speed of 28 ℃ and 180rpm to obtain first-class seed liquid; Inoculate the first-class seed liquid with the inoculum amount of 5% by volume percentage to house 100ml liquid seed culture medium In a 500mL Erlenmeyer flask, culture at 28°C and 180rpm for 96 hours to obtain a fermentation broth; centrifuge the fermentation broth at 5000rpm for 5min at 5000rpm, wash and centrifuge three times with distilled water, discard the supernatant and place the bacteria in a constant temperature drying oven...

Embodiment 2

[0040] The embodiment of the present invention provides a method of using Schizochytrium to promote the fermentation of Bacillus subtilis to produce N-acetylneuraminic acid, which is roughly the same as the steps in Example 1, the difference is that in this embodiment, 0h (that is, when inoculating) add 2g / L of the above-mentioned Schizochytrium fungus powder.

Embodiment 3

[0042] The embodiment of the present invention provides a method of using Schizochytrium to promote the fermentation of Bacillus subtilis to produce N-acetylneuraminic acid, which is roughly the same as the steps in Example 1, the difference is that in this embodiment, 0h (that is, when inoculating) add 3g / L of the above-mentioned Schizochytrium fungus powder.

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Abstract

The invention relates to a method for producing N-acetylneuraminic acid by promoting fermentation of bacillus subtilis, and belongs to the field of production of N-acetylneuraminic acid through fermentation of bacillus subtilis. According to the method for producing N-acetylneuraminic acid by promoting fermentation of bacillus subtilis, the bacillus subtilis capable of producing the N-acetylneuraminic acid through fermentation is inoculated to a fermentation medium for fermentation and culture, and in the fermentation and culture process, schizochytrium limacinum powder is added. According tothe method for producing the N-acetylneuraminic acid by promoting fermentation of bacillus subtilis, the yield of the N-acetylneuraminic acid can be effectively increased.

Description

technical field [0001] The invention relates to the technical field of producing N-acetylneuraminic acid by fermentation of Bacillus subtilis, and in particular to a method for promoting the production of N-acetylneuraminic acid by fermentation of Bacillus subtilis. Background technique [0002] N-acetylneuraminic acid (N-acetylneuraminic acid) Neu5Ac is the first contact site for cell information transmission, and its molecular structure is diverse, so Neu5Ac is involved in multiple physiological processes such as cell recognition, signal transduction, tumorigenesis, and fertilization , Neu5Ac can also regulate the anti-inflammatory activity of IgG, enhance infant immunity, affect the integrity, permeability and activity of nerve cells, and promote the development of infant brain, so the production of N-acetylneuraminic acid has attracted more attention and attention. Research. [0003] N-acetylneuraminic acid has great economic value, and there are many production methods...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P19/26C12R1/125C12R1/645
CPCC12P19/26C12P39/00
Inventor 朱薇薇陈祥松李翔宇吴金勇袁丽霞姚建铭
Owner 武汉中科光谷绿色生物技术有限公司
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