Method for promoting the production of N-acetylneuraminic acid by fermentation of Bacillus subtilis
A technology of acetylneuraminic acid and Bacillus subtilis, which is applied in the field of Bacillus subtilis fermentation to produce N-acetylneuraminic acid, can solve the problems of difficult to meet the needs of use and low fermentation yield, and achieve the effect of increasing yield
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Embodiment 1
[0032] The embodiment of the present invention provides a method of using Schizochytrium to promote the fermentation of Bacillus subtilis to produce N-acetylneuraminic acid, which mainly includes the following steps:
[0033]S11. Cultivate Schizochytrium, take out the Schizochytrium strains preserved in glycerol tubes from the -80°C freezer, and transfer 0.3mL of Schizochytrium strains to the In the 250mL Erlenmeyer flask of 50mL liquid seed culture medium, cultivate 48h under the rotating speed of 28 ℃ and 180rpm to obtain first-class seed liquid; Inoculate the first-class seed liquid with the inoculum amount of 5% by volume percentage to house 100ml liquid seed culture medium In a 500mL Erlenmeyer flask, culture at 28°C and 180rpm for 96 hours to obtain a fermentation broth; centrifuge the fermentation broth at 5000rpm for 5min at 5000rpm, wash and centrifuge three times with distilled water, discard the supernatant and place the bacteria in a constant temperature drying oven...
Embodiment 2
[0040] The embodiment of the present invention provides a method of using Schizochytrium to promote the fermentation of Bacillus subtilis to produce N-acetylneuraminic acid, which is roughly the same as the steps in Example 1, the difference is that in this embodiment, 0h (that is, when inoculating) add 2g / L of the above-mentioned Schizochytrium fungus powder.
Embodiment 3
[0042] The embodiment of the present invention provides a method of using Schizochytrium to promote the fermentation of Bacillus subtilis to produce N-acetylneuraminic acid, which is roughly the same as the steps in Example 1, the difference is that in this embodiment, 0h (that is, when inoculating) add 3g / L of the above-mentioned Schizochytrium fungus powder.
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