Thermophilic sporotrichum YM-2, compound bacterial agent thereof, and application of compound bacterial agent

A thermophilus lateralis, YM-2 technology, applied in the field of microorganisms, can solve problems such as increased production cost, low enzyme activity, strains that are not resistant to high temperature, etc., to avoid pollution, stable strain performance, and high viable count. Effect

Active Publication Date: 2019-11-08
QINGDAO AGRI UNIV +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current industrial production of cellulase cannot be produced in summer in many places because the strains are not resistant to high temperature, and the activity of the produced enzyme is low, which increases the production cost and limits the application of cellulase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thermophilic sporotrichum YM-2, compound bacterial agent thereof, and application of compound bacterial agent
  • Thermophilic sporotrichum YM-2, compound bacterial agent thereof, and application of compound bacterial agent
  • Thermophilic sporotrichum YM-2, compound bacterial agent thereof, and application of compound bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Screening and Identification of Sporotrichum thermophile YM-2

[0025] 1. Screening of Sporotrichum thermophila YM-2

[0026] Taking soil as a sample, add 1g of soil to 10mL sterile water and mix well to make 10 -1 Diluent, draw 10 -1 Diluent 1mL was added to 9mL sterile water and mixed to make 10 -2 diluent, and so on to make 10 -3 、10 -4 、10 -5 Diluent, take 10 -3 、10 -4 、10 -5 The diluted solution was spread on the PDA medium, cultured at 45°C for 6 days, and the obvious colonies were selected for multiple purifications to obtain a single colony, which was named YM-2, and stored in the PDA slant medium. The formula of described PDA culture medium is: potato juice 1000mL, glucose 10g, agar 20g, pH6.8; Wherein the preparation method of potato juice is: get the potato 200g of peeling, cut into fritters, add water 1000mL and after boiling 30min, The potato pieces were filtered off, and the filtrate was made up to 1000 mL.

[0027] Described bacterial...

Embodiment 2

[0031] Embodiment 2: the preparation of Dispensorium thermophila YM-2 composite bacterial agent

[0032] The compound bacterial agent of Disporosporum thermophila of the present invention comprises the following components: the bacteria content is 4×10 8 ~8×10 8 CFU / g Sporidium thermophila, 1×10 8 ~3×10 8 CFU / g of thermophilic bacillus, 1×10 8 ~3×10 8 CFU / g of thermophilic actinomycetes, 1×10 8 ~2×10 8 CFU / g of Candida utilis and 1 x 10 8 ~2×10 8CFU / g Lactobacillus plantarum, the carrier is soybean protein powder and cake; among them, it is necessary to ensure that the effective number of viable bacteria in the compound bacterial agent is not less than 5×10 8 CFU / g.

[0033] In this example, except Dispospora thermophila, other strains are commercially available products.

Embodiment 3

[0034] Embodiment 3: the application of described composite bacterial agent in broiler breeding

[0035] The composite bacterial agent prepared in Example 2 was added to the broiler feed at a mass ratio of 0.1%, and used after compacting, sealing and fermenting for 5 days.

[0036] Select 100 healthy 1-day-old broilers with no obvious difference in body weight and randomly divide them into 2 groups: the bacterial agent group and the control group. Each group has 2 replicates, and each replicate group has 25 chickens. Before feeding, the chicken coop, chicken coop and surrounding environment are disinfected, and the chicken coop is raised in a cage mode. The chicken coop has an automatic temperature control heating device. During the feeding process, the broilers in the bacterial agent group were fed with feed that had been fermented with compound bacterial agents, while the broilers in the control group were fed with feed without any compound bacterial agents; when feeding, th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a thermophilic sporotrichum YM-2, a compound bacterial agent thereof, and application of the compound bacterial agent. The bacterial strain is preserved in the general microbiology center of China Committee for Culture Collection of Microorganisms on August 27, 2019 with the preservation number being CGMCC 18127. The colony of the bacterial strain is white, large in shape, loose in texture, flocculent, dry in appearance, thin in mycelium and in an arbuscular shape; and the growth temperature and pH of the bacterial strain are wide, the suitable temperature is 42 DEG C-48DEG C, and the suitable pH is 5.0-7.0. The invention further provides the compound bacterial agent made of the thermophilic sporotrichum YM-2, and application of the compound bacterial agent in preparation of additives for production of single cell protein in broiler culture, organic fertilizer maturity, cucumber planting and waste residue fermentation. The compound bacterial agent has the characteristics of high number of living bacteria, high adaptability of the bacterial strain, stable bacterial performance, simple preparation method and low costs, and has broad application prospects.

Description

technical field [0001] The invention mainly relates to the technical field of microbes, in particular to a strain of Sporosporium thermophila YM-2 and its composite bacterial agent and application. Background technique [0002] Cellulose is the most abundant renewable resource in the world, but because of the complexity of its structure and the straight-chain macromolecular compound in which glucose molecules are bound by β-1,4 glycosidic bonds, it is difficult to decompose, so the current research on cellulose effective use is still rare. Cellulase is a general term for enzymes that degrade cellulose, including three main components, namely endoglucanase, exoglucanase and β-glucosidase. Cellulase produced by microorganisms can convert cellulose into various products in various fields that humans need, so this enzyme system has broad application prospects in food, detergent, textile and feed industries, such as in food In industry, cellulase is used to degrade natural cell...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K10/18A23K50/75A01B79/02C12R1/645
CPCA01B79/02A23K10/18A23K50/75C12N1/14C12N1/145C12R2001/645
Inventor 赵春梅庄会树赵秀杰钟士传王瑞
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap