Thermophilic sporotrichum YM-2, compound bacterial agent thereof, and application of compound bacterial agent
A thermophilus lateralis, YM-2 technology, applied in the field of microorganisms, can solve problems such as increased production cost, low enzyme activity, strains that are not resistant to high temperature, etc., to avoid pollution, stable strain performance, and high viable count. Effect
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Embodiment 1
[0024] Example 1: Screening and Identification of Sporotrichum thermophile YM-2
[0025] 1. Screening of Sporotrichum thermophila YM-2
[0026] Taking soil as a sample, add 1g of soil to 10mL sterile water and mix well to make 10 -1 Diluent, draw 10 -1 Diluent 1mL was added to 9mL sterile water and mixed to make 10 -2 diluent, and so on to make 10 -3 、10 -4 、10 -5 Diluent, take 10 -3 、10 -4 、10 -5 The diluted solution was spread on the PDA medium, cultured at 45°C for 6 days, and the obvious colonies were selected for multiple purifications to obtain a single colony, which was named YM-2, and stored in the PDA slant medium. The formula of described PDA culture medium is: potato juice 1000mL, glucose 10g, agar 20g, pH6.8; Wherein the preparation method of potato juice is: get the potato 200g of peeling, cut into fritters, add water 1000mL and after boiling 30min, The potato pieces were filtered off, and the filtrate was made up to 1000 mL.
[0027] Described bacterial...
Embodiment 2
[0031] Embodiment 2: the preparation of Dispensorium thermophila YM-2 composite bacterial agent
[0032] The compound bacterial agent of Disporosporum thermophila of the present invention comprises the following components: the bacteria content is 4×10 8 ~8×10 8 CFU / g Sporidium thermophila, 1×10 8 ~3×10 8 CFU / g of thermophilic bacillus, 1×10 8 ~3×10 8 CFU / g of thermophilic actinomycetes, 1×10 8 ~2×10 8 CFU / g of Candida utilis and 1 x 10 8 ~2×10 8CFU / g Lactobacillus plantarum, the carrier is soybean protein powder and cake; among them, it is necessary to ensure that the effective number of viable bacteria in the compound bacterial agent is not less than 5×10 8 CFU / g.
[0033] In this example, except Dispospora thermophila, other strains are commercially available products.
Embodiment 3
[0034] Embodiment 3: the application of described composite bacterial agent in broiler breeding
[0035] The composite bacterial agent prepared in Example 2 was added to the broiler feed at a mass ratio of 0.1%, and used after compacting, sealing and fermenting for 5 days.
[0036] Select 100 healthy 1-day-old broilers with no obvious difference in body weight and randomly divide them into 2 groups: the bacterial agent group and the control group. Each group has 2 replicates, and each replicate group has 25 chickens. Before feeding, the chicken coop, chicken coop and surrounding environment are disinfected, and the chicken coop is raised in a cage mode. The chicken coop has an automatic temperature control heating device. During the feeding process, the broilers in the bacterial agent group were fed with feed that had been fermented with compound bacterial agents, while the broilers in the control group were fed with feed without any compound bacterial agents; when feeding, th...
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