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A kind of abamectin culture medium with slow-release effect and preparation method thereof

A technology of abamectin and medium, which is applied in the direction of fungi, etc., can solve the problems that the fermentation efficiency cannot meet the requirements, it is not suitable for large-scale industrial production, and there is no separate competition for the characteristic medium of abamectin.

Active Publication Date: 2019-04-30
青岛海科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the fermentation unit of industrial production of abamectin is mostly 4000~5000ug / ml, and the fermentation unit of some scientific research institutions in the laboratory can reach 8500ug / ml, but it is not suitable for large-scale industrial production. Enterprises with higher production levels can It reaches about 7300 ug / ml, but the fermentation efficiency still cannot meet the requirements
Although there are many types of culture media used for industrial fermentation, there is no culture medium developed solely for the characteristics of abamectin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] First prepare the slow-release components by preparing the components according to the mass ratio of polyethylene glycol: sodium propionate: water: porous silicon dioxide of 1:3:7:8, specifically: polyethylene glycol and sodium propionate Dissolve in water, add silicon dioxide for adsorption, and then air-dry at a temperature of 40°C. Then, the non-sustained-release ingredients wheat flour, soybean cake powder, yeast powder, dephenolized cottonseed protein powder, ammonium sulfate, calcium carbonate, and cobalt chloride dihydrate are in a mass ratio of 8:1:1:1:0.1:0.01:0.01 Prepare. The slow-release component and the non-slow-release component are evenly mixed and stirred according to the ratio of 1:500, and the fineness of the powder is 80 mesh, and the fermentation unit of the obtained abamectin is 7650ug / ml.

Embodiment 2

[0014] First prepare the slow-release components by preparing the components according to the mass ratio of polyethylene glycol: sodium propionate: water: porous silicon dioxide of 1.5:4:8:12, specifically: polyethylene glycol and sodium propionate Dissolve in water, add silicon dioxide for adsorption, and then air-dry at a temperature of 55°C. Then, the non-sustained-release ingredients wheat flour, soybean cake powder, yeast powder, dephenolized cottonseed protein powder, ammonium sulfate, calcium carbonate, and cobalt chloride dihydrate are in a mass ratio of 9:1.5:1.5:1.3:0.15:0.02:0.02 Prepare. The slow-release component and the non-slow-release component are evenly mixed and stirred according to the ratio of 2:500, and the fineness of the powder is 130 mesh, and the fermentation unit of the obtained avermectin is 7750ug / ml.

Embodiment 3

[0016] First prepare the slow-release components by preparing the components according to the mass ratio of polyethylene glycol: sodium propionate: water: porous silica of 2:5:10:15, specifically: polyethylene glycol and sodium propionate Dissolve in water, add silicon dioxide for adsorption, and then air-dry at a temperature of 70°C. Then, the non-sustained-release ingredients wheat flour, soybean cake powder, yeast powder, dephenolized cottonseed protein powder, ammonium sulfate, calcium carbonate, and cobalt chloride dihydrate are in a mass ratio of 10:2:2:1.5:0.2:0.03:0.03 Prepare. The slow-release component and the non-slow-release component are evenly mixed and stirred according to the ratio of 3:500, and the fineness of the powder is 200 mesh, and the fermentation unit of the obtained abamectin is 7700ug / ml.

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Abstract

The invention provides a medium with slow release effects for avermectin and a preparation method thereof, and belongs to the field of biological fermentation. The medium mainly comprises the following components: yeast powder, wheat powder, soybean cake powder, dephenolized cottonseed protein powder, ammonium sulfate, calcium carbonate, CoCl2.2H2O, and a slow-release component; nutrient compositions are reasonably prepared and can be reasonably and fully utilized, and demands for growing and breeding bacterial strains are satisfied. The slow-release component is composed of polyethylene glycol, sodium propionate and silica; and the slow-release component can keep appropriate concentration of fermentation liquor, so that generation of the target product is promoted, and intracellular metabolites are promoted to release out of cells, and output of the target product is improved. The medium product can effectively improve fermentation titer of avermectin, content of residual nutrient compositions in the fermentation waste liquid is small, raw materials are saved, and cost for treating waste water is reduced.

Description

technical field [0001] The invention belongs to the field of biological fermentation, and in particular relates to an abamectin culture medium with slow-release effect and a preparation method thereof. Background technique [0002] Avermectin is a macrolide antibiotic insecticide with contact and ingestion toxicity. It can be used as both agricultural insecticide and veterinary insecticide. It has broad-spectrum, high-efficiency and Excellent characteristics of being safe and biodegradable to humans, non-target animals and the environment. Due to its novel chemical structure, unique mechanism of action, high efficiency and low toxicity, abamectin has good application value and market prospects in the production of medicine, agriculture and animal husbandry. [0003] At present, the fermentation unit of industrial production of abamectin is mostly 4000~5000ug / ml, and the fermentation unit of some scientific research institutions in the laboratory can reach 8500ug / ml, but it ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 张术臻梁小田李俊芳
Owner 青岛海科生物技术有限公司
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