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Enzymatic hydrolysis buffer solution for promoting acetone-butanol-ethanol fermentation production of fibers and application thereof

A technology of buffer solution and enzymatic hydrolysis solution, which is applied in the field of lignocellulose resource utilization, can solve problems such as the inability to realize the promotion and improvement of butanol metabolism, and achieve the effect of promoting efficient production and simplifying the preparation scheme

Pending Publication Date: 2018-12-18
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the addition of acetic acid / sodium acetate buffered saline solution in the enzymatic hydrolysis process can promote the metabolism of acetone to a certain extent, it cannot promote and improve the metabolism of butanol

Method used

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  • Enzymatic hydrolysis buffer solution for promoting acetone-butanol-ethanol fermentation production of fibers and application thereof
  • Enzymatic hydrolysis buffer solution for promoting acetone-butanol-ethanol fermentation production of fibers and application thereof
  • Enzymatic hydrolysis buffer solution for promoting acetone-butanol-ethanol fermentation production of fibers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Example 1: Using acetic acid / butyric acid dilute solution to prepare fiber enzymatic hydrolysis buffer and realize enzymatic saccharification and ABE fermentation of sugarcane straw residue after ethylenediamine pretreatment

[0117] (1) Rinse the juiced sugarcane stalks with deionized water until there is no residual free sugar on the surface, and dry at 105° C. to absolute dryness. Then crush until the particle size is below 40 mesh. The sugarcane straw residue was fully mixed with a pre-prepared 10% (w / v) aqueous solution of ethylenediamine, and pretreated at 120° C. for 1 hour. Afterwards, the reactor was cooled to room temperature, and centrifuged at 10000 rpm to obtain solid sugarcane stalk pretreatment residue. Rinse the pretreatment residue with deionized water until the surface is neutral. The obtained neutral pretreatment residue was baked to absolute dryness at 105°C. The measured solid yield was 49.8%, and the contents of cellulose, hemicellulose and lign...

Embodiment 2

[0124] Example 2: Utilizing the organic acid in the ABE fermentation broth to prepare an enzymatic hydrolysis buffer, and carrying out batch ABE fermentation.

[0125] Get the ABE fermentation broth in Example 1, and suspend steaming at 45° C. under 20 kPa until the volume of the concentrated fermentation broth is half of the original fermentation broth. After that, add deionized water to the decompressed concentrated fermented liquid, so that the volume of the fermented liquid remains consistent with the volume before concentration. The concentration of ethanol in the diluted concentrated fermented liquid detected by gas chromatography is 0.6 g / L, the concentrations of acetic acid and butyric acid are 2.5 g / L and 1.5 g / L, and the pH of the fermented liquid is 4.3.

[0126]Add concentrated ammonia water to the diluted concentrated fermented liquid to adjust the pH to 5. Afterwards, the neutral sugarcane stalk residue obtained by pretreatment and washing with the ethylenediami...

Embodiment 3

[0127] Example 3: Using different concentrations of butyric acid / ammonium butyrate enzymatic hydrolysis buffer to enzymolyze wheat straw and carry out free ABE fermentation

[0128] (1) The wheat straw was crushed until the particle size was below 40 meshes, and then fully mixed with 5% (w / v) dilute sulfuric acid prepared in advance, and pretreated at 120°C for 1 hour. Afterwards, the reactor was cooled to room temperature and centrifuged at 10000 rpm to obtain solid wheat straw pretreatment residue. Rinse the residue with deionized water until the surface is neutral. The resulting neutral residue was dried to absolute dryness at 105°C. 4 groups in parallel.

[0129] (2) Add 25mM, 50mM, 75mM and 100mM butyric acid / ammonium butyrate buffer solution to the 4 groups of pretreatment residues obtained by the reaction respectively according to the solid addition amount of 10% (w / v), the pH of the buffer solution is 4.8 . After thorough mixing, 30 FPU / g of residual cellulase was ...

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Abstract

The invention relates to an enzymatic hydrolysis buffer solution for promoting acetone-butanol-ethanol fermentation production of fibers and an application thereof. The enzymatic hydrolysis buffer solution is prepared from butyric acid and other organic weak acids metabolized by butanol-producing clostridium and cations provided by hydroxides of metals necessary for microbial metabolism or ammoniawater, has the pH of 4-7, and is suitable for enzymatic hydrolysis of lignocellulose by cellulase; after enzymatic hydrolysis is completed, acetone-butanol-ethanol fermentation production of the fibers can be realized by adding alkaline substances to adjust the pH without supply of other exogenous nutrients. The invention provides a simplified preparation scheme of the enzymatic hydrolysis buffersolution; the problem of inhibition on the acetone-butanol-ethanol fermentation by a traditional citric acid / sodium citrate buffer solution is effectively solved, and the acetone-butanol-ethanol metabolic flow is affected by addition of intermediate products such as acetic acid and butyric acid in the enzymatic hydrolysis process, and thus the high-efficiency production of solvents is promoted.

Description

technical field [0001] The invention belongs to the technical field of lignocellulose resource utilization, and in particular relates to an enzymolysis buffer for promoting the fermentation and production of fiber acetone butanol and ethanol and an application thereof. Background technique [0002] Butanol is an important bulk chemical and a potential alternative fuel, widely used in industry, food, medicine and other fields. Using lignocellulosic raw materials to produce bio-based n-butanol through acetone-butanol-ethanol (ABE) fermentation has the advantages of renewable and environmental friendliness, and has become a research hotspot in recent years. However, except for the high product inhibition in ABE fermentation, butanol-producing microorganisms were more sensitive to the external environment and stimuli. [0003] In the process route of converting lignocellulose into bio-based n-butanol, lignocellulose is depolymerized by pretreatment means. After depolymerization...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P7/16C12P7/28C12R1/145
CPCC12P7/10C12P7/16C12P7/28C12P2203/00Y02E50/10
Inventor 谭天伟蔡的张长伟秦培勇陈长京曾海旺周明园
Owner BEIJING UNIV OF CHEM TECH
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