A Strain of Disposporum thermophila ym-2 and Its Composite Bacterial Agent and Its Application

A thermophilus lateralis and compound bacterial agent technology, applied in the field of microorganisms, can solve the problems of high temperature resistance, increased production cost, low enzyme activity, etc., and achieves high viable bacteria count, avoids pollution, and stabilizes bacterial performance. Effect

Active Publication Date: 2020-09-22
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current industrial production of cellulase cannot be produced in summer in many places because the strains are not resistant to high temperature, and the activity of the produced enzyme is low, which increases the production cost and limits the application of cellulase

Method used

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  • A Strain of Disposporum thermophila ym-2 and Its Composite Bacterial Agent and Its Application
  • A Strain of Disposporum thermophila ym-2 and Its Composite Bacterial Agent and Its Application
  • A Strain of Disposporum thermophila ym-2 and Its Composite Bacterial Agent and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Screening and Identification of Sporotrichum thermophile YM-2

[0025] 1. Screening of Sporotrichum thermophila YM-2

[0026] Taking soil as a sample, add 1g of soil to 10mL sterile water and mix well to make 10 -1 Diluent, draw 10 -1 Diluent 1mL was added to 9mL sterile water and mixed to make 10 -2 diluent, and so on to make 10 -3 、10 -4 、10 -5 Diluent, take 10 -3 、10 -4 、10 -5 The diluted solution was spread on the PDA medium, cultured at 45°C for 6 days, and the obvious colonies were selected for multiple purifications to obtain a single colony, which was named YM-2, and stored in the PDA slant medium. The formula of described PDA culture medium is: potato juice 1000mL, glucose 10g, agar 20g, pH6.8; Wherein the preparation method of potato juice is: get the potato 200g of peeling, cut into fritters, add water 1000mL and after boiling 30min, The potato pieces were filtered off, and the filtrate was made up to 1000 mL.

[0027] Described bacterial...

Embodiment 2

[0031] Embodiment 2: the preparation of Dispensorium thermophila YM-2 composite bacterial agent

[0032] The compound bacterial agent of Disporosporum thermophila of the present invention comprises the following components: the bacteria content is 4×10 8 ~8×10 8 CFU / g Sporidium thermophila, 1×10 8 ~3×10 8 CFU / g of thermophilic bacillus, 1×10 8 ~3×10 8 CFU / g of thermophilic actinomycetes, 1×10 8 ~2×10 8 CFU / g of Candida utilis and 1 x 10 8 ~2×10 8CFU / g Lactobacillus plantarum, the carrier is soybean protein powder and cake; among them, it is necessary to ensure that the effective number of viable bacteria in the compound bacterial agent is not less than 5×10 8 CFU / g.

[0033] In this example, except Dispospora thermophila, other strains are commercially available products.

Embodiment 3

[0034] Embodiment 3: the application of described composite bacterial agent in broiler breeding

[0035] The composite bacterial agent prepared in Example 2 was added to the broiler feed at a mass ratio of 0.1%, and used after compacting, sealing and fermenting for 5 days.

[0036] Select 100 healthy 1-day-old broilers with no obvious difference in body weight and randomly divide them into 2 groups: the bacterial agent group and the control group. Each group has 2 replicates, and each replicate group has 25 chickens. Before feeding, the chicken coop, chicken coop and surrounding environment are disinfected, and the chicken coop is raised in a cage mode. The chicken coop has an automatic temperature control heating device. During the feeding process, the broilers in the bacterial agent group were fed with feed that had been fermented with compound bacterial agents, while the broilers in the control group were fed with feed without any compound bacterial agents; when feeding, th...

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Abstract

The present invention provides a strain of Sporosporium thermophila YM‑2 and its compound bacterial agent and its application. The strain was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on August 27, 2019, and the preservation number is: CGMCC 18127 . The colonies of this strain are white, large in shape, loose in texture, flocculent in appearance, dry in appearance, and thin in mycelia in arbuscular shape; the growth temperature and pH of this strain are relatively wide, and the suitable temperature is 42°C~48°C. The suitable pH is 5.0~7.0. The present invention also provides a composite bacterial agent prepared by using Disposporium thermophila YM-2 and the application of the composite bacterial agent in the preparation of additives for broiler breeding, organic manure decomposing, cucumber planting and waste residue fermentation to produce single-cell protein , the composite bacterial agent has the characteristics of high number of viable bacteria, strong strain adaptability, stable strain performance, simple preparation method, low cost, etc., and has broad application prospects.

Description

technical field [0001] The invention mainly relates to the technical field of microbes, in particular to a strain of Sporosporium thermophila YM-2 and its composite bacterial agent and application. Background technique [0002] Cellulose is the most abundant renewable resource in the world, but because of the complexity of its structure and the straight-chain macromolecular compound in which glucose molecules are bound by β-1,4 glycosidic bonds, it is difficult to decompose, so the current research on cellulose effective use is still rare. Cellulase is a general term for enzymes that degrade cellulose, including three main components, namely endoglucanase, exoglucanase and β-glucosidase. Cellulase produced by microorganisms can convert cellulose into various products in various fields that humans need, so this enzyme system has broad application prospects in food, detergent, textile and feed industries, such as in food In industry, cellulase is used to degrade natural cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23K10/18A23K50/75A01B79/02C12R1/645
CPCA01B79/02A23K10/18A23K50/75C12N1/14C12N1/145C12R2001/645
Inventor 赵春梅庄会树赵秀杰钟士传王瑞
Owner QINGDAO AGRI UNIV
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