Burkholderia cepacia and application thereof

A technology of Burkholderia and onion, applied in applications, bacteria, fungicides, etc., can solve the problems of inability to be absorbed and utilized by plants, low utilization rate of phosphorus fertilizer, etc., and achieve significant bacteriostatic effect, improve soil conditions, and effect obvious effect

Active Publication Date: 2019-01-11
XINYANG NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in our country, the utilization rate of phosphorus fertilizer is extremely low, only 5-10%, and after more than 95% of phosphorus fertilizer is applied, it will be destroyed by Ca in the soil in a short period of time. 2+ , Fe 3+ 、Al 3+ Plasma fixation, forming insoluble minerals such as calcium phosphate / iron / aluminum, which cannot be absorbed and utilized by plants

Method used

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  • Burkholderia cepacia and application thereof
  • Burkholderia cepacia and application thereof
  • Burkholderia cepacia and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Screening of Efficient Phosphorus Solubilizing Strains

[0029] 1) Isolation of high-efficiency phosphorus-solubilizing strains

[0030] By microbiological methods, the rhizosphere soil was taken from a hundred-year-old tea tree in Xinyang City for microbial isolation. Weighed 1g of soil sample, put it into a 2mL centrifuge tube, added 1mL of sterile water, mixed well, and diluted to 10 -6 . Take 100 μL of bacterial suspension, spread it on the surface of insoluble phosphorus medium (inorganic phosphorus or organic phosphorus), and culture at 37°C for 3 to 5 days. On the surface of the culture medium, pick a bacterial strain with a large transparent circle diameter and high transparency. As an alternative bacterial strain, get a single bacterial colony and culture it on the beef extract peptone medium (beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 15.0g, distilled water 1000mL, pH 7.2-7.4).

[0031] Described inorganic phosphorus medium fo...

Embodiment 2

[0036] The identification of embodiment 2 phosphate solubilizing strain WY6-5

[0037] (1) Morphological identification of WY6-5

[0038] WY6-5 obtained a single colony after culturing in NA medium, the colony was milky white, opaque, with regular edges, smooth and moist surface, sticky and protruding colonies, Gram staining negative ( figure 2 ).

[0039] (2) Molecular biological identification of WY6-5

[0040]WY6-5 was cultured in NB culture medium (beef extract 3.0g, peptone 10.0g, NaCl 5.0g, distilled water 1000mL, pH7.2-7.4) for 48h, collected bacteria, and used tris-HCl (Amresco Company) and EDTA (Amresco Company) ) method to extract genomic DNA, phenol-chloroform-isoamyl alcohol (25:24:1) extraction, TER dissolution. The 16S rDNA was amplified with specific primers, cloned into Escherichia coli, picked a single colony, and sent to the company for sequencing. The length of the sequencing sequence is 1414bp, and the sequence number is SEQ ID NO.1.

[0041] The sequ...

Embodiment 3

[0051] Phosphorus Solubilizing Effect Analysis of Example 3 Burkholderia cepacia WY6-5

[0052] (1) Phosphorus solubilizing effect of phosphate solubilizing strain WY6-5 cultured in inorganic phosphorus liquid medium

[0053] Culture strain WY6-5 in NB culture medium (3.0g beef extract, 10.0g peptone, 5.0g NaCl, 1000mL distilled water, pH 7.2-7.4), 200rpm, shake culture at 37°C for 24h, centrifuge to collect bacteria, sterile water Wash 2 times, adjust the concentration to 10 8 cfu / mL, inoculated into 40mL inorganic phosphorus liquid medium ((NH 4 ) 2 SO 4 0.5g, MgSO 4 0.3g, NaCl 0.3g, Ca 3 (PO 4 ) 2 8.0g, glucose 10.0g, MnSO 4 (11%) 1 mL, FeSO 4 (1%) 1mL, water 1L, pH 7.2), with the inorganic phosphorus liquid medium inoculated with the same amount of sterile water as a control, at 37 ° C, 200rpm shaker culture. Each treatment was repeated 3 times, respectively at T 0 , T 3 , T 6 , T 12 , T 20 Samples were taken at time points, filtered through a filter memb...

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Abstract

The invention discloses a Burkholderia cepacia onion (Burkholderia cepacia) WY6-5 and an application thereof, deposit No.: CCTCC M 2018248. The onion Burkholderia cepacia WY6-5 has good application inphosphorus degradation, bacteriostasis, plant growth promotion and preparation of dimethyl disulfide.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to Burkholderia cepacia and applications thereof. Background technique [0002] China is a large agricultural production country, but also a large food production and consumption country. Soil fertility is of great significance to the stability of food production and social development. For a long time, due to excessive reliance on chemical fertilizers, the soil composition has been unbalanced, resulting in soil acidification, compaction, and salinization, which have seriously restricted the growth of grain production and the improvement of quality. Improving the soil environment and improving soil fertility are the focus of research at present and even in the future for a long time. To this end, in 2015, the Ministry of Agriculture of China formulated the "Action Plan for Zero Growth in Chemical Fertilizer Use by 2020", which plans to achieve zero growth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N31/02A01P3/00A01P21/00C05F11/08C12R1/01
CPCA01N31/02A01N63/00C05F11/08C12N1/205C12R2001/01
Inventor 宫安东朱梓钰万海燕吕翠华董飞燕魏芬芬廖玉才张静柏程琳张在宝袁红雨
Owner XINYANG NORMAL UNIVERSITY
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