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Method and kit for detecting atrazine, chlorpyrifos and triadimefon based on SERS technology

A detection kit and atrazine technology, applied in the field of pesticide detection, can solve the problems of low detection sensitivity, achieve high stability, and overcome the effect of long preparation cycle

Active Publication Date: 2021-02-02
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the current development status of SERS immunolabeling technology, in order to realize the simultaneous detection of a variety of pesticide small molecules, on the one hand, it is necessary to further develop the multi-component labeling technology of SERS, screen suitable marker molecules with different Raman characteristic signals, and prepare highly sensitive and multi-component pesticides. Component-labeled probes; on the other hand, study the detection mode of SERS immunolabeling technology to overcome the technical defect of low detection sensitivity caused by the immuno-sandwich method with few active sites for binding small molecules

Method used

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  • Method and kit for detecting atrazine, chlorpyrifos and triadimefon based on SERS technology

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preparation example Construction

[0038] In some embodiments, the preparation method of the artificial antigen of atrazine, chlorpyrifos, triadimefon comprises:

[0039] The hapten of the pesticide molecule is activated and reacted with NHS and EDC in turn, and then coupled with the carrier protein;

[0040] The pesticide molecule hapten is selected from any one of atrazine, chlorpyrifos and triadimefon.

[0041] In some embodiments, the molar ratio of the pesticide molecule hapten to NHS and EDC is 1: (1.7-2.3): (2.7-3.3);

[0042] In some embodiments, the molar ratio of the pesticide molecule hapten to NHS and EDC is 1:2:3.

[0043] In some embodiments, the carrier protein is BSA.

[0044] According to another aspect of the present invention, the present invention also relates to a method for detecting pesticides using the above kit, the method is used to detect one or more of atrazine, chlorpyrifos, triadimefon, the method include:

[0045] The substance to be detected is used to competitively bind to t...

Embodiment 1

[0048] 1. Synthesis of nano gold sol

[0049] First heat 300mL 0.01% chloroauric acid solution to boiling, quickly add a certain amount of 1% trisodium citrate aqueous solution, and obtain nano-gold sols with different particle sizes by changing the amount of sodium citrate added. Some blue at first, then light blue, then blue, then heated to red, boiled for 7 to 10 minutes to appear transparent orange-red, wherein the molar ratio of trisodium citrate to the chloroauric acid is 4.3.

[0050] 2. Preparation of signal-labeled probes based on gold nanospheres (AuNPs) / carrier protein / target

[0051] Triazophos hapten carrier protein, atrazine hapten carrier protein and chlorpyrifos hapten carrier protein were respectively selected, and covalent bond, electrostatic self-assembly and other reconstruction techniques were used to prepare gold nanospheres (AuNPs) / carrier protein / Three different signal-labeled probes composed of targets.

[0052] (1) Triazophos hapten (15mmol L -1 ,...

Embodiment 2

[0062] 1. Synthesis of nano gold sol

[0063] First heat 300mL 0.01% chloroauric acid solution to boiling, quickly add a certain amount of 1% trisodium citrate aqueous solution, and obtain nano-gold sols with different particle sizes by changing the amount of sodium citrate added. Some blue at first, then light blue, then blue, red after heating, and transparent orange-red after boiling for 7-10 minutes. In the experiment, it was found that when the molar ratio of trisodium citrate: chloroauric acid was 4.9, it was possible to synthesize gold nanoparticles of about 16nm. Stable storage for more than half a year.

[0064] 2. Preparation of signal-labeled probes based on gold nanospheres (AuNPs) / carrier protein / target

[0065] Triazophos hapten carrier protein, atrazine hapten carrier protein and chlorpyrifos hapten carrier protein were respectively selected, and covalent bond, electrostatic self-assembly and other reconstruction techniques were used to prepare gold nanospheres...

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Abstract

The invention relates to the field of pesticide detection, in particular to a method and kit for detecting atrazine, chlorpyrifos and triadimefon based on SERS technology. The kit is used to detect one or more of atrazine, chlorpyrifos, and triadimefon, and includes a solid phase carrier and a labeled probe; the solid phase carrier is coated with atrazine, chlorpyrifos, Molecularly imprinted polymer film of triadimefon; the labeled probe is a metal nanoparticle coupled with an artificial antigen and a labeled molecule; the artificial antigen is one of the artificial antigens of atrazine, chlorpyrifos, and triadimefon species, and the labeled molecules on each labeled probe are such that they can be distinguished from one another when detected using SERS techniques. The invention combines molecular imprinting technology and Raman signal amplification probe technology to establish a detection method for simultaneous detection of three pesticides.

Description

technical field [0001] The invention relates to the field of pesticide detection, in particular to a method and kit for detecting atrazine, chlorpyrifos and triadimefon based on SERS technology. Background technique [0002] Surface-enhanced Raman spectroscopy (SERS) technology, as a new spectral analysis technology, is widely used in the detection of pharmaceutical analysis, biological analysis, environmental pollutants and other fields due to its advantages of simple operation and fast detection speed. In recent years, SERS has shown great application value in the field of rapid real-time detection of pesticide residues. There have been many related research reports on the detection of pesticides by SERS, but these detection methods largely depend on the relationship between the adsorbed molecules and the SERS substrate. The interaction and the surface plasmon resonance effect of the SERS active substrate. Therefore, the sensitivity, stability, and uniformity of the SERS ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 佘永新颜朦朦王静王淼王珊珊郑鹭飞曹振邵勇
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS