A controllable rotary operating device and method for an optical tweezers system

Abstract

The invention relates to a controllable rotary operation device of an optical tweezers system. The device comprises an optical tweezers generation module, a polarization state control module, a polarization state detection module, a sample stage, and an illumination and imaging module, wherein a linear polarization state Gaussian laser beam is focused by a high numerical aperture objective to forma light trap near the sample stage and stably capture the binary heterogeneous sphere; the polarization state control module is used to adjust a laser polarization state azimuth so as to control thesynchronous rotation of the captured particles around the optical axis; the polarization detection module is used to solve the laser polarization state at the light trap during the entire rotating operation; and the position and orientation of the controlled binary heterogeneous sphere are saved and recorded in real time by the illumination and imaging module. The operational steps carried out using the device described are also given. The device provided by the invention is simple and convenient to operate, and can not only control the continuous rotation of the binary heterogeneous sphere, but also control the stop of the rotation at any time to stabilize in any orientation.

Description

technical field [0001] The invention relates to a controllable rotation operation method of an optical tweezers system. In particular, the controllable rotation operation of the linearly polarized Gaussian optical trap on the binary heterogeneous sphere is realized. Background technique [0002] As an important tool in the field of single-molecule force spectroscopy, optical tweezers have played a huge role in probing the structure and function of biological macromolecules such as nucleic acids and proteins. Traditional optical tweezers can only achieve three-dimensional translation, that is, they can only stretch biomolecules but cannot rotate them. However, it is well known that the spatial structure of biomolecules is extremely complex, the double helix structure of deoxyribonucleic acid, the α helix structure in the secondary structure of proteins, and the coiled coil in the tertiary structure all involve rotation operations. Traditional optical tweezers can only stret...

AI Technical Summary

Problems solved by technology

This technology has developed rapidly in recent years, but its application in the field of single-molecule force spectroscopy is still limited due to the destruction of the surface biomolecular structure due to the high local temperature of the microspheres.

In addition, the above two technologies can only make the controlled particles rotate continuously, but cannot keep the particles in a certain state, and the rotation direction and rotation speed are not controlled. These shortcomings limit their application in the field of single molecules.

Images