Method for simultaneously detecting various residues of veterinary drug in meat food

Abstract

The invention relates to the technical field of the detection of residues of veterinary drug, and provides a method for simultaneously detecting various residues of veterinary drug in meat food. The method comprises the following steps of treating a sample, establishing a standard curve and calculating the content of each residue of veterinary drug in the detected sample, and the detection methodis an HPLC-MS (Ultra Performance Liquid Chromatography-Mass Spectrometry) method. Therefore, a PLS-A (Probabilistic Latent Semantic Analysis) solid phase extraction small column is adopted, activationand balance steps are not required, more than 95% of matrix disturbance substances, including protein, salt, phospholipid and the like, can be removed after extraction liquid directly passes throughthe small column, most veterinary drugs can directly pass without being retained, and therefore, different categories of residues of veterinary drug can be completely extracted only through preliminary treatment for one time. A preliminary treatment step is simplified, a solid phase extraction column does not need to be activated, in addition, various categories of residues of veterinary drug canbe simultaneously detected, preliminary treatment time is greatly shortened, and detection cost is lowered. A result indicates that the method is accurate, quick, convenient and reliable in quantitative results and is very suitable for simultaneously processing multiple batches of samples.

Description

technical field [0001] The invention relates to the technical field of detection of animal residues, in particular to a method for simultaneous detection of various animal residues in meat products. Background technique [0002] The veterinary drugs used more in animal husbandry in my country mainly include sulfonamides, quinolones, tetracyclines, β-receptor agonists, chloramphenicol, malachite greens, sodium pentachlorophenate and other compounds. The abuse of these drugs can easily cause serious pollution of livestock products, and enter into muscle and other animal-derived foods through metabolic pathways, and finally accumulate in the human body through the food chain, and these drugs have side effects such as causing allergic reactions and making the human body develop drug resistance. Therefore, monitoring the residues of various veterinary drugs in meat products has positive significance for ensuring food safety. [0003] At present, the simultaneous detection method...

AI Technical Summary

Problems solved by technology

[0003] At present, the simultaneous detection methods of multiple veterinary drug residues reported in relevant standards and literature are mostly divided into several detection methods according to the types of veterinary drugs. The process is cumbersome, the recovery rate is low, and it is impossible to detect multiple veterinary drug residues at the same time

[0004] In summary, there are obviously inconveniences and defects in the actual use of the existing technology, so it is necessary to improve

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