A kind of preparation method of high-purity low-density lipoprotein

Abstract

The invention belongs to the technical field of material extraction, and particularly relates to a preparation method of a high purity and low density lipoprotein. Chylomicrons and very low density lipoproteins in blood plasma are centrifuged and removed according to different densities, remain blood plasma is extracted and added withpotassium bromide and the density is adjusted to 1.045g / mL, centrifugation is carried out again to obtain a normal low density lipoprotein located at the uppermost layer, and a phosphate buffer salt solution is used for dialyzing to reduce K+ concentration; and aresultof the K+ concentration of the low density lipoprotein before and after dialyzing measured by a full-automatic biochemical analyzer is compared with a result measured by an osmotic pressure analyzer, if the K+ concentration of the low density lipoprotein is dialyzed to be with no obvious differences with the osmotic pressure of the phosphate buffer salt solution, the potassium bromide is considered to be completely dialyzed, and then the high purity and low density lipoprotein is obtained. The preparation method of the high purity and low density lipoprotein can obtain the high purity and low density lipoprotein to meet of existing experimentalrequirements.

Description

technical field [0001] The invention belongs to the technical field of substance extraction, and in particular relates to a preparation method of high-purity low-density lipoprotein. Background technique [0002] Blood lipids are the general term for cholesterol, triglycerides and lipids in serum. Blood lipids are insoluble in water and must be combined with apolipoproteins to form lipoproteins to dissolve in blood and be transported to tissues for metabolism. Low-density lipoprotein cholesterol (Low-density Lipoprotein Cholesterol, LDL-C) is the lipoprotein with the most cholesterol content (about 50%) in the blood, so the concentration of LDL-C can basically reflect the total amount of blood low-density lipoprotein (LDL). In addition, LDL can be oxidized to oxidized LDL (Oxidized Low-density Lipoprotein, Ox-LDL). [0003] Three common ultracentrifugation methods for lipoprotein analysis are: analytical ultracentrifugation, zonal ultracentrifugation, and preparative ultrac...

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Problems solved by technology

In addition, many laboratory dialysis bags are recycled after cleaning, sterilization and other steps. Repeated treatment of dialysis bags will significantly affect the efficiency of dialysis.

The amount of routine extraction of plasma LDL is generally small. For example, when a large amount of LDL is required in the experiment, the amount of blood collected from a single individual cannot meet the requirements.

However, multiple blood collections from a single individual, a single blood collection from multiple individuals, or multiple blood collections from multiple individuals cannot guarantee the inter-batch difference in LDL activity, let alone the stability and repeatability of the experimental results.

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