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Hepatitis C virus antigen fluorescent immunochromatography detection kit and preparation method therefor

A technology of hepatitis C virus and fluorescence immunochromatography, applied in measurement devices, analytical materials, instruments, etc., can solve problems such as difficulty in promotion, inability to quantify colloidal gold reagents, and easy false positives, and achieve good detection accuracy. , The effect of good detection precision and good detection sensitivity

Pending Publication Date: 2019-02-12
XIAMEN TONGRENXIN BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nucleic acid detection requires high equipment and technology, and is prone to false positives, so it is difficult to promote in routine and grassroots laboratories
Antigen detection reagents are mostly based on ELISA, chemiluminescence or colloidal gold, such as the ELISA kit of Ortho Company in the United States, the ELISA detection kit of Hunan Jingda Pharmaceutical Co., Ltd., and the chemiluminescence hepatitis C virus core antigen (HCV Core Ag ) detection kits, etc., but both ELISA and chemiluminescence need to be carried out in the central testing laboratory, and colloidal gold reagents cannot be quantified

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A fluorescent immunochromatographic detection kit for hepatitis C virus antigen. The fluorescent immunochromatographic detection kit includes the following components: fluorescent microsphere-labeled hepatitis C virus antibody 1, hepatitis C virus antibody 2, goat anti-mouse Capture antibody (control line antibody), phosphate buffer, and sample diluent.

[0032] Among them, the sample diluent includes the following concentrations of components: Tris-HCl buffer 100ml / L, CHAPS dry powder 15g / L, Triton X-1003ml / L and SDS 150g / L; the concentration of Tris-HCl buffer is 1mol / L , the pH is 8.0; the concentration of the phosphate buffer is 0.01mol / L, and the pH is 7.2.

[0033] The preparation method of the above-mentioned fluorescence immunochromatography detection kit comprises the following steps:

[0034] (1) Prepare the sample diluent: take 100ml 1mol / L Tris-HCl buffer solution, 15g CHAPS dry powder, 3ml Triton X-100 and 150g SDS and mix them evenly in a container, then ...

Embodiment 2

[0040] A fluorescent immunochromatographic detection kit for hepatitis C virus antigen. The fluorescent immunochromatographic detection kit includes the following components: fluorescent microsphere-labeled hepatitis C virus antibody 1, hepatitis C virus antibody 2, goat anti-mouse Capture antibody (control line antibody), phosphate buffer, and sample diluent.

[0041] Among them, the sample diluent includes the following concentrations of components: Tris-HCl buffer 100ml / L, CHAPS dry powder 15g / L, Triton X-1003ml / L and SDS 150g / L; the concentration of Tris-HCl buffer is 1mol / L , the pH is 8.0; the concentration of the phosphate buffer is 0.01mol / L, and the pH is 7.3.

[0042] The preparation method of the above hepatitis C virus antigen fluorescence immunochromatography detection kit, the preparation method of the fluorescence immunochromatography detection kit comprises the following steps:

[0043] (1) Prepare sample diluent: Take 100ml 1mol / L Tris-HCl buffer solution, 15...

Embodiment 3

[0049] A fluorescent immunochromatographic detection kit for hepatitis C virus antigen. The fluorescent immunochromatographic detection kit includes the following components: fluorescent microsphere-labeled hepatitis C virus antibody 1, hepatitis C virus antibody 2, goat anti-mouse Capture antibody (control line antibody), phosphate buffer, and sample diluent.

[0050] Among them, the sample diluent includes the following concentrations of components: Tris-HCl buffer 100ml / L, CHAPS dry powder 15g / L, Triton X-1003ml / L and SDS 150g / L; the concentration of Tris-HCl buffer is 1mol / L , the pH is 8.0; the concentration of the phosphate buffer is 0.01mol / L, and the pH is 7.4.

[0051] The preparation method of the above hepatitis C virus antigen fluorescence immunochromatography detection kit, the preparation method of the fluorescence immunochromatography detection kit comprises the following steps:

[0052] (1) Prepare sample diluent: Take 100ml 1mol / L Tris-HCl buffer solution, 15...

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Abstract

The invention discloses a hepatitis C virus antigen fluorescent immunochromatography detection kit and a preparation method therefor. The detection kit comprises the following components: a fluorescent microsphere labeled hepatitis C virus antibody 1, a hepatitis C virus antibody 2, a goat anti-mouse capture antibody, a phosphate buffer, and a sample diluent. The preparation method for the detection kit comprises the following steps: preparing the sample diluent; preparing the fluorescent microsphere labeled hepatitis C virus antibody 1; preparing a carrier 1 coated with the fluorescent microsphere labeled hepatitis C virus antibody 1; preparing a carrier 2 coated with the hepatitis C virus antibody 2 and the goat anti-mouse capture antibody; and assembling the standard substance to obtainthe fluorescent immunochromatography detection kit. The detection kit can rapidly and conveniently carry out quantitative detection on hepatitis C virus antigens on site, and has good detection sensitivity, precision and accuracy.

Description

technical field [0001] The invention relates to the technical field of immune detection and analysis, in particular to a hepatitis C virus antigen fluorescent immunochromatographic detection kit and a preparation method thereof. Background technique [0002] Hepatitis C is caused by the hepatitis C virus (HCV), which is spread through blood. At present, there are more than 40 million hepatitis C virus-infected patients in China, among which 70% of hepatitis C patients develop chronic hepatitis, 20% develop liver cirrhosis, and 12% develop liver cancer, which is seriously harmful. Therefore, early detection, early diagnosis, and early treatment are of great significance for hepatitis C patients. [0003] Common early detection methods for hepatitis C virus include nucleic acid detection and antigen detection. Nucleic acid testing requires high equipment and technology, and is prone to false positives, so it is difficult to promote in routine and grassroots laboratories. An...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/569G01N33/533
CPCG01N33/533G01N33/558G01N33/56983
Inventor 周国栋
Owner XIAMEN TONGRENXIN BIO-TECH CO LTD
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