Hepatitis C virus antigen fluorescent immunochromatography detection kit and preparation method therefor
A technology of hepatitis C virus and fluorescence immunochromatography, applied in measurement devices, analytical materials, instruments, etc., can solve problems such as difficulty in promotion, inability to quantify colloidal gold reagents, and easy false positives, and achieve good detection accuracy. , The effect of good detection precision and good detection sensitivity
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Embodiment 1
[0031] A fluorescent immunochromatographic detection kit for hepatitis C virus antigen. The fluorescent immunochromatographic detection kit includes the following components: fluorescent microsphere-labeled hepatitis C virus antibody 1, hepatitis C virus antibody 2, goat anti-mouse Capture antibody (control line antibody), phosphate buffer, and sample diluent.
[0032] Among them, the sample diluent includes the following concentrations of components: Tris-HCl buffer 100ml / L, CHAPS dry powder 15g / L, Triton X-1003ml / L and SDS 150g / L; the concentration of Tris-HCl buffer is 1mol / L , the pH is 8.0; the concentration of the phosphate buffer is 0.01mol / L, and the pH is 7.2.
[0033] The preparation method of the above-mentioned fluorescence immunochromatography detection kit comprises the following steps:
[0034] (1) Prepare the sample diluent: take 100ml 1mol / L Tris-HCl buffer solution, 15g CHAPS dry powder, 3ml Triton X-100 and 150g SDS and mix them evenly in a container, then ...
Embodiment 2
[0040] A fluorescent immunochromatographic detection kit for hepatitis C virus antigen. The fluorescent immunochromatographic detection kit includes the following components: fluorescent microsphere-labeled hepatitis C virus antibody 1, hepatitis C virus antibody 2, goat anti-mouse Capture antibody (control line antibody), phosphate buffer, and sample diluent.
[0041] Among them, the sample diluent includes the following concentrations of components: Tris-HCl buffer 100ml / L, CHAPS dry powder 15g / L, Triton X-1003ml / L and SDS 150g / L; the concentration of Tris-HCl buffer is 1mol / L , the pH is 8.0; the concentration of the phosphate buffer is 0.01mol / L, and the pH is 7.3.
[0042] The preparation method of the above hepatitis C virus antigen fluorescence immunochromatography detection kit, the preparation method of the fluorescence immunochromatography detection kit comprises the following steps:
[0043] (1) Prepare sample diluent: Take 100ml 1mol / L Tris-HCl buffer solution, 15...
Embodiment 3
[0049] A fluorescent immunochromatographic detection kit for hepatitis C virus antigen. The fluorescent immunochromatographic detection kit includes the following components: fluorescent microsphere-labeled hepatitis C virus antibody 1, hepatitis C virus antibody 2, goat anti-mouse Capture antibody (control line antibody), phosphate buffer, and sample diluent.
[0050] Among them, the sample diluent includes the following concentrations of components: Tris-HCl buffer 100ml / L, CHAPS dry powder 15g / L, Triton X-1003ml / L and SDS 150g / L; the concentration of Tris-HCl buffer is 1mol / L , the pH is 8.0; the concentration of the phosphate buffer is 0.01mol / L, and the pH is 7.4.
[0051] The preparation method of the above hepatitis C virus antigen fluorescence immunochromatography detection kit, the preparation method of the fluorescence immunochromatography detection kit comprises the following steps:
[0052] (1) Prepare sample diluent: Take 100ml 1mol / L Tris-HCl buffer solution, 15...
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