The present application relates to the technical field of
protein detection, and particularly relates to an ELISA detection kit for quantitatively detecting S-palmitoylation modified target proteins and a use method thereof; the kit comprises
Protein G magnetic beads, a 96-well transparent
polystyrene microwell plate, a 96-well plate magnetic frame, solution A, solution B, solution C, solution D, solution E, solution F,
solution G; the present application relates to the comprehensive application of
immunoprecipitation (IP),
click chemistry and
enzyme-linked immunosorbent (ELISA)
signal amplification experimental principles, and realizes the effect of high-
throughput quantitative detection of S-palmitoylation modified target proteins, is suitable for the mechanism and function research of S-palmitoylation modified target proteins of various samples (such as high-
throughput screening of
small molecule inhibitors targeting S-palmitoylation modified target proteins), and improves the experimental efficiency.