Method for rapidly detecting phenyllactic acid content by using reversed-phase high performance liquid chromatography

A technology of reversed-phase high performance liquid phase and phenyllactic acid, which is applied in the field of high performance liquid chromatography analysis, can solve the problems of high labor and preparation costs, shortened detection time, long detection time, etc., and achieves accurate and reliable detection results, mobile phase and instrumentation. The effect of low requirements and fast peak time

Inactive Publication Date: 2019-05-21
SHANGHAI OCEAN UNIV
View PDF9 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of high instrument requirements, long detection time, high labor and preparation costs and low sensitivity when detecting phenyllactic acid at present, the purpose of the present invention is to provide a method for quickly detecting the content of phenyllact

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly detecting phenyllactic acid content by using reversed-phase high performance liquid chromatography
  • Method for rapidly detecting phenyllactic acid content by using reversed-phase high performance liquid chromatography
  • Method for rapidly detecting phenyllactic acid content by using reversed-phase high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1

[0033] Using reversed-phase high performance liquid chromatography to quickly detect the content of phenyllactic acid, the specific steps are:

[0034] Step a. Configure the mobile phase, filter, degas, adjust the instrument, and balance the chromatographic column;

[0035] Step b. Use the phenyllactic acid standard solution prepared by Shanghai Ziqi Biotechnology Co., Ltd.'s phenyllactic acid standard product, and dilute successively to obtain the concentration of 10.0μg / mL, 5.0μg / mL, 2.0μg / mL, 1.0μg / mL, 0.5μg / mL of standard solution, the phenyllactic acid standard solution was analyzed by high performance liquid chromatograph. The standard solution of each concentration was injected twice, and the data was processed by Agilent 1260 offline data processing software. The chromatogram is as follows figure 1 As shown in the figure, the retention time of 6.253min is the peak of phenyllactic acid, and the rest are impurity peaks. The peak area is shown in Table 1. The...

Example Embodiment

[0039] Example 2

[0040] In order to verify the feasibility and accuracy of the above-mentioned Example 1, a precision test was performed. The test used a standard solution of phenyllactic acid with a concentration of 2.0 μg / mL and injected 8 consecutive samples. The chromatographic conditions were exactly the same as those in the example. Table 2 shows.

[0041] Table 2: Precision test values

[0042] Serial number

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for rapidly detecting a phenyllactic acid content by using reversed-phase high performance liquid chromatography and belongs to the food detection technology field. Chromatographic conditions comprise that a C18 chromatographic column (4.6*250 mm, 5mum) is included; a mobile phase A is a 0.05% trifluoroacetic acid water solution; a mobile phase B is acetonitrile, and the volume ratio of mobile phases A and B is 3:1; a flow rate is 1 mL/min; a sample size is 10 muL; a chromatographic column temperature is 20-30 DEG C; and a detection wavelength is 210 nm. Impurities and phenyllactic acid in a sample to be tested are separated, the content of the phenyllactic acid is detected, and qualitative and quantitative operation is performed on the phenyllactic acid through retention time and a peak area. The operation of the method is simple, detection is rapid, cost is low, qualitative and quantitative operation is accurate, separation with an impurity peak can be achieved, and the method is suitable for the rapid batch detection of a total phenyllactic acid amount in food and a fermentation solution.

Description

technical field [0001] The invention belongs to the technical field of high-performance liquid chromatography analysis, and relates to the rapid detection of phenyllactic acid content in food and fermented liquid, in particular to a method for rapidly detecting the content of phenyllactic acid by reverse-phase high-performance liquid chromatography. Background technique [0002] Phenyllactic acid is a new type of antibacterial substance produced by lactic acid bacteria. It has broad-spectrum antibacterial properties and can inhibit the growth of a variety of food-borne pathogens and spoilage bacteria, such as Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Salmonella, etc. Phenyllactic acid occurs naturally in honey, yeast dough, and Chinese pickles. [0003] At present, the detection of phenyllactic acid mainly includes thin-layer chromatography and high-performance liquid chromatography. The detection limit of thin-layer chromatography is high and the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/89
Inventor 欧杰张雯李柏林谢佳雨
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap