Method for error correction in transgenic organism quantitative determination

A quantitative detection and transgenic technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of different nucleic acid volumes, differences, biases, etc., to achieve high sensitivity, ensure accuracy and accuracy , Eliminate the effect of difference interference data analysis

Active Publication Date: 2016-01-20
NAT INST OF METROLOGY CHINA
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Problems solved by technology

However, the quality of powder weighed and the volume of nucleic acid extracted each time may be different or there may be differences. It may be biased to compare only with the concentration of nucleic acid.

Method used

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  • Method for error correction in transgenic organism quantitative determination
  • Method for error correction in transgenic organism quantitative determination
  • Method for error correction in transgenic organism quantitative determination

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0070] Quantitative detection of components in rice powder

[0071] 1. Materials and methods

[0072] 1. The sample to be tested is the standard substance of rice seed powder of transgenic rice Kechidao No. 2 with a mass fraction of 2% (developed by China Institute of Metrology)

[0073] 2. Accurately weigh the sample to be tested and the quality control product (the standard substance of the transgenic rice Kemodao No. 2 rice seed powder with a mass fraction of 1% and 5% content) with a balance

[0074] 3. Using the Plant Genome Extraction Kit GenomicDNAPurificationKit (Promega, USA) extracts the genomic DNA of the sample to be tested and the quality control product, and uses a UV spectrophotometer and a Picogreen kit to determine the purity and concentration of the extracted DNA, respectively.

[0075] 4. The standard (containing about 10 6 、10 5 、10 4 、10 3 、10 2 Copy / ml Plasmid Molecular Standard Substances of Kechidao 2 and RBE4 Gene Fragment) and the DNA of the s...

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Abstract

The invention relates to a method for error correction in transgenic organism quantitative determination. According to the method for error correction, a ratio of nucleic acid extraction ratios of a transgenic organism to a receptor non-transgenic organism is calculated and is used as a correction coefficient and real and accurate content of the transgenic component is obtained according to the correction coefficient.

Description

[0001] The present invention is a divisional application of the patent application with the application number 2012101041601 and the invention name "Nucleic Acid Quantitative Detection Kit for Genetically Transgenic Rice Kemodao No. 2". Technical field: [0002] The invention relates to a method for correcting the deviation between the transgene detection value and the real value in the quantitative detection of transgenic organisms, specifically, a method for establishing a correction coefficient for calculating the correction deviation. Background technique: [0003] GMO detection first requires the extraction of DNA from transgenic plants. Theoretically speaking, the DNA extraction efficiency of transgenic plants and recipient plants should be the same. However, after some organisms insert foreign genes to form genetically modified organisms, some characteristics of the genetically modified organisms will change, so the genome of the genetically modified organisms will be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6806C12Q1/6851C12Q2537/165C12Q2531/113C12Q2545/101
Inventor 王晶隋志伟李亮余笑波臧超董莲华
Owner NAT INST OF METROLOGY CHINA
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