Selective medium for quantitative determination of V.anguillarum and its preparation
A technology for quantitative detection and culture medium, applied in the field of selective culture medium and its preparation, can solve the problems of complex operation, high technical and equipment requirements, and achieve the effect of reducing losses
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[0009] Example 1
[0010] Medium composition: sorbitol 15g; yeast powder 4g; NaCl 35g; ampicillin 5mg; cresol red 40mg; bromothymol blue 40mg; agar 15g; distilled water 1000ml.
[0011] Add all ingredients together except ampicillin and heat and agitate to dissolve; after dissolving, cool to 50°C, adjust pH to 8.6 (± 0.2) with 5M NaOH, then add ampicillin; pour medium into plates After cooling, place it upside down in a 15°C incubator; the storage time should not exceed 3 weeks. Vibrio eel M3 colonies were yellow on this medium.
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[0012] Example 2
[0013] Medium composition: sorbitol 15g; yeast powder 5g; NaCl 30g; ampicillin 5mg; cresol red 40mg; bromothymol blue 40mg; agar 15g; distilled water 1000ml.
[0014] All ingredients, except ampicillin, are added together and stirred with heat to dissolve. After dissolving, cool it to 50°C, adjust the pH to 8.6 with 4M NaOH, and then add ampicillin; pour the medium into the plate, put it upside down in a 15°C incubator after cooling; the storage time should not exceed 3 weeks. Vibrio eel M3 colonies were yellow on this medium.
Example Embodiment
[0015] Example 3
[0016] Medium composition: sorbitol 15g; yeast powder 3g; NaCl 35g; ampicillin 5mg; cresol red 40mg; bromothymol blue 40mg; agar 18g; distilled water 1000ml.
[0017] Add all ingredients together except ampicillin and heat and agitate to dissolve; after dissolving, cool to 50°C, adjust the pH to 8.6 with 5M NaOH, then add ampicillin; pour the medium into the plate, after cooling Place upside down in a 15°C incubator; keep for no longer than 3 weeks. Vibrio eel M3 colonies were yellow on this medium.
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