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Selective medium for quantitative determination of V.anguillarum and its preparation

A technology for quantitative detection and culture medium, applied in the field of selective culture medium and its preparation, can solve the problems of complex operation, high technical and equipment requirements, and achieve the effect of reducing losses

Inactive Publication Date: 2006-05-10
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the disadvantages of high technology and equipment requirements and complicated operation in the quantitative detection of Vibrio anguillaris in the prior art, the present invention provides a selective medium that can quantitatively and rapidly detect Vibrio anguillarum M3

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Medium composition: sorbitol 15g; yeast powder 4g; NaCl 35g; ampicillin 5mg; cresol red 40mg; bromomusmol blue 40mg; agar 15g; distilled water 1000ml.

[0011] Except for ampicillin, add all ingredients together and heat and stir to dissolve; after dissolving, cool to 50°C, adjust the pH to 8.6 (±0.2) with 5M NaOH, then add ampicillin; pour the medium into the plate After cooling, place it upside down in a 15°C incubator; the storage time should not exceed 3 weeks. Vibrio anguillarum M3 colonies were yellow on this medium.

Embodiment 2

[0013] Medium composition: sorbitol 15g; yeast powder 5g; NaCl 30g; ampicillin 5mg; cresol red 40mg; bromomusmol blue 40mg; agar 15g; distilled water 1000ml.

[0014] Add all ingredients together and heat with agitation, except the ampicillin, to dissolve. After dissolving, cool to 50°C, adjust the pH to 8.6 with 4M NaOH, and then add ampicillin; pour the medium into the plate, cool and place it upside down in a 15°C incubator; the storage time should not exceed 3 weeks. Vibrio anguillarum M3 colonies were yellow on this medium.

Embodiment 3

[0016] Medium composition: sorbitol 15g; yeast powder 3g; NaCl 35g; ampicillin 5mg; cresol red 40mg; bromomusmol blue 40mg; agar 18g; distilled water 1000ml.

[0017] Except for ampicillin, add all the ingredients together and heat and stir to dissolve; after dissolving, cool to 50°C, adjust the pH to 8.6 with 5M NaOH, then add ampicillin; pour the medium into the plate, and after cooling Place it upside down in a 15°C incubator; the storage time should not exceed 3 weeks. Vibrio anguillarum M3 colonies were yellow on this medium.

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PUM

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Abstract

The invention discloses a selective culture medium for the quantitative determination of Vibrio anguillarum and its preparing process, wherein the culture medium comprises sorbic alcohol 15g, yeast powder or yeast grease 2-5g, NaCl 20-60g, ampictllin 5mg, cresol red 40mg, bromothymol blue 40mg, gelose 12-20g, and distilled water 1000ml.

Description

technical field [0001] The invention relates to a microbial culture medium, in particular to a selective culture medium for quantitatively detecting Vibrio anguillarum M3 in cultured seawater and its preparation. Background technique [0002] The method that Vibrio anguillarum is carried out quantitative detection in seawater has colony hybridization (Aoki, T., I.Hirono, T.de Castro, and T.Kitao.1989.Rapid identification of Vibrio anguillarum by colony hybridization.J.Appl.Ichthyol. 5:67-73.), southern hybridization (Martinez-Picado, J., A.R Blanch, and J.Jofre.1994.Rapid detection and identification of Vibrio anguillarum by using a specific oligonucleotide probecomplementary to 16S rRNA.Appl.Environ.Microbiol. 60: 732-737. and Rehnstam, A.-S., A. Norqvist, H. Wolf-Watz, and A. Hagstrom. 1989. Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe .Appl.Environ.Microbiol.55:1907-1910.), but the method o...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 邹玉霞莫照兰张培军徐永立王春玲张振冬肖鹏
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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