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Ratiometric fluorescent probe and application thereof in visual detection of glutathione

A ratiometric fluorescent probe, glutathione technology, applied in the direction of fluorescence/phosphorescence, luminescent materials, measuring devices, etc., can solve the problems of insufficient selectivity and sensitivity, unfavorable rapid and real-time monitoring, and cumbersome processing procedures. Achieve excellent sensitivity, fast response, and solve unstable effects

Active Publication Date: 2020-06-12
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Common instrument detection methods include gas chromatography, high performance liquid chromatography, mass spectrometry, etc., which can be used to achieve quantitative detection to some extent, but these methods have certain disadvantages, such as heavy equipment, cumbersome sample pretreatment process and It takes a long time, and the selectivity and sensitivity are not good enough, which is not conducive to fast and real-time monitoring. Therefore, it is necessary to develop an efficient, convenient and fast detection method to realize the detection of glutathione

Method used

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  • Ratiometric fluorescent probe and application thereof in visual detection of glutathione
  • Ratiometric fluorescent probe and application thereof in visual detection of glutathione
  • Ratiometric fluorescent probe and application thereof in visual detection of glutathione

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Preparation of blue carbon dots

[0038] Dissolve 1.725g of L-proline in 20mL of ultrapure water, transfer it to a 50mL polytetrafluoroethylene reactor after dissolving completely, react at 200°C for 6h, cool to room temperature, and use the intercepted carbon dot solution The dialysis bag with a molecular weight of 1000 was dialyzed for 48 hours, and then stored in a refrigerator at 4°C.

[0039] 2. Preparation of orange luminescent gold clusters

[0040] Before starting the preparation, soak all the glass instruments and magnetic stirring bars used in the experiment with aqua regia for 30-120 minutes, and then wash them with ultrapure water several times until they are clean and ready for use. Add 3mL of 10mM fresh Prepared glutathione solution, 0.2mL of 100mM chloroauric acid solution, 6.8mL of ultrapure water, stirred vigorously at 25°C for 5 minutes, then raised the temperature to 70°C and stirred slowly for 24 hours until the solution turned light yellow, Sto...

Embodiment 2

[0048] 1. Preparation of blue carbon dots

[0049] The implementation process of this step is the same as in Example 1.

[0050] 2. Preparation of orange luminescent gold clusters

[0051] The implementation process of this step is the same as in Example 1.

[0052] 3. Construction of ratiometric fluorescent probes

[0053] The implementation process of this step is the same as in Example 1.

[0054] 4. Orange fluorescence of copper ion quenching ratiometric fluorescent probe

[0055] The implementation process of this step is the same as in Example 1.

[0056] 5. Quantitative detection of glutathione in water by the ratiometric fluorescent probe to the prepared ratiometric probe and Cu(NO 3 ) 2 Add 0, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 μM glutathione to the mixed system in turn, and after 5 minutes, use a fluorescence spectrometer to monitor the blue signal at 412nm and the orange signal at 602nm The fluorescence intensity of the signal was established by esta...

Embodiment 3

[0060] 1. Preparation of blue carbon dots

[0061] The implementation process of this step is the same as in Example 1.

[0062] 2. Preparation of orange luminescent gold clusters

[0063] The implementation process of this step is the same as in Example 1.

[0064] 3. Construction of ratiometric fluorescent probes

[0065] The implementation process of this step is the same as in Example 1.

[0066] 4. Orange fluorescence of copper ion quenching ratiometric fluorescent probe

[0067] The implementation process of this step is the same as in Example 1.

[0068] 5. Quantitative detection of glutathione in water by ratiometric fluorescent probe

[0069] The implementation process of this step is the same as in Example 2.

[0070] 6. Selectivity test of ratio probe

[0071] Such as Figure 5 As shown, to the ratio probe and Cu(NO 3 ) 2 No interfering medium was added to the mixed system, and a concentration of 100 μM inorganic ions (Na + ,K + , Ca 2+ ,Fe 2+ ), gluco...

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Abstract

The invention discloses a ratiometric fluorescent probe and application thereof in visual detection of glutathione. The ratiometric fluorescent probe is a synthetic system constructed on the basis ofblue carbon dots and orange gold nanocluster dual-channel fluorescence signals. A fluorescence quenching system constructed by combining the ratiometric probe with copper ions can be used for fluorescence enhancement detection of glutathione; wherein a blue fluorescence signal of the ratiometric probe serves as an internal standard and keeps stable, quenched orange fluorescence can be recovered byglutathione, and quantitative monitoring of glutathione is achieved by measuring the linear relation between the ratio of the orange fluorescence intensity to the blue fluorescence intensity and theglutathione concentration. According to the method disclosed by the invention, visual detection of glutathione can be achieved by utilizing only one handheld ultraviolet lamp, and the method has the characteristics of rapid reaction, high sensitivity, good selectivity, strong anti-interference capability, low detection limit and the like.

Description

technical field [0001] The invention relates to a ratio fluorescent probe and its application in visual detection of glutathione, which can be used for quantitative monitoring of glutathione levels in human serum by constructing a ratio fluorescent probe fluorescence-enhanced visual detection. Background technique [0002] With the improvement of human living standards, people pay more and more attention to health. Many major diseases often have no obvious symptoms in the early stage but are accompanied by changes in the level of some biomolecules. Therefore, a method for quantitative monitoring of small biomolecules is urgently needed. As the most abundant non-protein thiol compound in human biological systems, glutathione plays a key role in various cellular functions such as gene expression and signal transmission. Abnormalities in glutathione have been linked to a variety of human diseases such as Alzheimer's, cancer, aging, AIDS and Parkinson's disease. The abnormal l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C09K11/58C09K11/02B82Y30/00G01N21/64
CPCC09K11/65C09K11/58C09K11/025B82Y30/00G01N21/6428G01N21/6447G01N2021/6432G01N2021/6439
Inventor 杨亮蒋长龙王振洋刘变化张淑东
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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