Determination of yeast RNA (Ribonucleic Acid) by photoluminescence of ruthenium-based metal complex

A ruthenium complex, yeast technology, applied in the field of photochemical sensing, to achieve the effect of simple quantitative detection

Inactive Publication Date: 2016-02-03
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been many DNA molecular optical switches based on ruthenium (II) complexes. However, the research on the interaction between ruthenium (II) complexes and RNA has mainly focused on the bonding properties and bonding modes. Molecular photoswitches rarely reported for RNA

Method used

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  • Determination of yeast RNA (Ribonucleic Acid) by photoluminescence of ruthenium-based metal complex
  • Determination of yeast RNA (Ribonucleic Acid) by photoluminescence of ruthenium-based metal complex
  • Determination of yeast RNA (Ribonucleic Acid) by photoluminescence of ruthenium-based metal complex

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: Complex [Ru(bpy) 2 (bipp)] (ClO 4 ) 2 、[Ru(bpy) 2 (bopp)] (ClO 4 ) 2 and [Ru(bpy) 2 (btpp)] (ClO 4 ) 2 Synthesis

[0012] Complex [Ru(bpy) 2 (bipp)] (ClO 4 ) 2 、[Ru(bpy) 2 (bopp)] (ClO 4 ) 2 and [Ru(bpy) 2 (btpp)] (ClO 4 ) 2 According to the method reported by our research group [Han, M.J.; Duan, Z.M.; Hao, Q.; Wang, K.Z.J.Phys.Chem.C2007, 111, 16577-16585.] Synthesis.

[0013] The ligands bipp, bopp and btpp are pressed by pyrazine[2,3-f][1,10]phenanthroline-2-carboxylic acid and o-phenylenediamine, 2-aminophenol and 2-aminothiophenol, respectively. The molar ratio of 1:1 is obtained by condensation reaction in polyphosphoric acid heated to 200°C. After the reaction was completed and cooled to room temperature, the mixture was poured into 400 mL of cold water with stirring, and the pH of the system was adjusted to 7 with concentrated ammonia water. The obtained precipitate was suction filtered to obtain a crude product, which was recrys...

Embodiment 2

[0017] Embodiment 2: Complex [Ru(bpy) 2 (bipp)] (ClO 4 ) 2 、[Ru(bpy) 2 (bopp)] (ClO 4 ) 2 and [Ru(bpy) 2 (btpp)] (ClO 4 ) 2 Determination of Yeast RNA by Photoluminescence and Drawing of Working Curve

[0018] Fluorescence emission spectra were measured on a Shimadzu RF-5301PC fluorescence spectrophotometer with an excitation wavelength of 450 nm.

[0019] [Ru(bpy) at a concentration of 4.6 μM 2 (bipp)] (ClO 4 ) 2 The yeast RNA solution was continuously added to the Tris-HCl (5mM, pH=7.1) solution, and the change of the emission spectrum was measured with the increase of the yeast RNA concentration until the emission spectrum of the complex did not change any more. When the concentration of yeast RNA reached 160μM, the emission spectrum of the complex did not change, and the intensity of the emission peak at 623nm gradually increased with the increase of RNA concentration, accompanied by a blue shift of 9nm. Wherein the concentration of yeast RNA is in the range of...

Embodiment 3

[0022] Embodiment 3: Complex [Ru(bpy) 2 (bipp)] (ClO 4 ) 2 、[Ru(bpy) 2 (bopp)] (ClO 4 ) 2 and [Ru(bpy) 2 (btpp)] (ClO 4 ) 2 Determining the detection limit of yeast RNA

[0023] Fluorescence emission spectra were measured on a Shimadzu RF-5301PC fluorescence spectrophotometer with an excitation wavelength of 450 nm.

[0024] Take 6 copies [Ru(bpy) 2 (bipp)] (ClO 4 ) 2 (4.6 μM) Tris-HCl (5 mM, pH=7.1) solution, the emission spectra of the solutions were measured respectively, and six parallel measurement results were obtained. The luminous intensity of the complex solution at 623nm was obtained from the emission spectrum measured each time, which were 8.138, 8.039, 8.291, 8.275, 8.141, and 8.342, and the standard deviation σ of the 6 luminous intensity values ​​was calculated 1 = 0.1064. [Ru(bpy) 2 (bipp)] (ClO 4 ) 2 Determination of the slope K of the working curve of yeast RNA 1 =5.52749μM -1 , using 3σ / K yielded a detection limit of 0.06 μM.

[0025] Take ...

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Abstract

The invention discloses a method for quantitatively determining the concentration of yeast RNA (Ribonucleic Acid) in a water solution with a fluorescent spectrometry by applying three types of ruthenium complexes. The method has high sensitivity.

Description

technical field [0001] The invention belongs to the field of photochemical sensing, and relates to a synthesis method of three similar polypyridine ruthenium (II) complexes and their application in related fields such as luminescent determination of yeast RNA. Background technique [0002] Nucleic acid (DNA and RNA) bases store a large amount of genetic information and are important components of organisms. They play a key role in protein biosynthesis and are closely related to life activities such as reproduction, growth and development of organisms. They are also cancers. Targets for disease diagnosis and chemotherapy drugs such as AIDS and AIDS. Therefore, the research and preparation of molecular probes that can specifically bind and measure nucleic acids are of great significance to the advancement of life sciences and medicine and the healthy development of the entire human race [Campisi, D.; Morii, T.; Barton, J.K.Biochemistry1994, 33, 4130.]. In the past decades, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王克志贾嘉韩美娇
Owner BEIJING NORMAL UNIVERSITY
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