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A method for defatting bone specimens

A bone and specimen technology, applied in the field of specimen preparation, can solve the problems such as the thoracic cage cannot keep its original shape, the compact bone layer is easily damaged, and the small bones are easily lost, so as to achieve convenient research and teaching, good degreasing effect, and ensure quality and safety. Effect

Active Publication Date: 2021-05-04
广州广宠生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chen Qihong and others boiled the soaked horse bones in soda water and clean water. After the cooking was completed, they were soaked in clean water while scraping off the soft tissues, and finally washed and dried. However, this method is laborious, labor-intensive and easy. Damage to the compact bone layer, easy to lose small bones, and the thorax cannot maintain its original shape
Fang Dianyu and others pointed out that sodium carbonate solution was used for cooking, and then the hot alkaline water in the pot was extracted with a metal syringe, injected from the drill hole, and the bone marrow in the bone was washed repeatedly, and then rinsed with warm water. Finally prepare to degrease, but this method is uneven in degreasing, easy to lose small bones, and more likely to damage bone
Gasoline or caustic soda is used for degreasing, which is dangerous and expensive; caustic soda is used for uneven degreasing and is more likely to damage bone

Method used

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  • A method for defatting bone specimens
  • A method for defatting bone specimens
  • A method for defatting bone specimens

Examples

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Effect test

Embodiment 1

[0040] Embodiment 1 A kind of method of bone specimen degreasing

[0041] A method for degreasing a bone specimen, comprising the following steps:

[0042] (1) Divide the bones into mesh bags, remove the flesh, and leave the clean bones. Use an electric drill to drill holes at both ends of the bones for larger bones, especially the long bones of the limbs, so as to remove the inside of the bones. bone marrow, etc., to effectively shorten the degreasing time; put the bones into 10% fur degreasing agent for preliminary degreasing, wherein the mass-volume ratio of bone and degreasing agent is 5kg:20L, after soaking for 48 hours, take it out, and use a pressure water gun (10~ 25MPa) to wash away impurities such as grease floating on the surface of the bone;

[0043] (2) Put the skeleton in a proper amount of methylal-acetone mixed solution prepared in a sealed rubber barrel, wherein the mass-volume ratio of the bone to the methylal-acetone mixed solution is 6kg:30L; the ratio of ...

Embodiment 2

[0045] Embodiment 2 A kind of method of bone specimen degreasing

[0046] A method for degreasing a bone specimen, comprising the following steps:

[0047] (1) Divide the bones into mesh bags, remove the flesh, and leave the clean bones. Use an electric drill to drill holes at both ends of the bones for larger bones, especially the long bones of the limbs, so as to remove the inside of the bones. bone marrow, etc., to effectively shorten the degreasing time; put the bones into 13% fur degreasing agent for preliminary degreasing, wherein the mass volume ratio of bone and degreasing agent is 4kg: 15L, after soaking for 50h, take it out, and use a pressure water gun to wash away the floating Oil and other impurities on the bone surface;

[0048] (2) Put the skeleton in a proper amount of methylal-acetone mixed solution prepared in a sealed rubber bucket, wherein the mass-volume ratio of the bone to the methylal-acetone mixed solution is 7kg:25L; The mass ratio is 1:1.5; then se...

Embodiment 3

[0050] Embodiment 3 A kind of method of bone specimen degreasing

[0051] A method for degreasing a bone specimen, comprising the following steps:

[0052](1) Divide the bones into mesh bags, remove the flesh, and leave the clean bones. Use an electric drill to drill holes at both ends of the bones for larger bones, especially the long bones of the limbs, so as to remove the inside of the bones. bone marrow, etc., to effectively shorten the degreasing time; put the bones into 15% degreasing agent for preliminary degreasing, wherein the mass-volume ratio of bone and degreasing agent is 7kg: 30L, after soaking for 45 hours, take it out, and rinse it with a pressure water gun Oil and other impurities on the bone surface;

[0053] (2) Put the bones in a sealed rubber barrel prepared with a proper amount of methylal-acetone mixed solution, wherein the mass-volume ratio of bone to acetal-acetone mixed solution is 6kg: 35L; the mass ratio of acetal to acetone Then seal the rubber b...

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Abstract

The invention discloses a method for defatting a bone specimen. The method includes the following steps: S1. After punching holes at both ends of the deboned bone, soak the bone in 10% to 15% degreasing agent for 45 to 50 hours, and use a pressure water gun to wash away the impurities floating on the surface of the bone. Impurities; S2. Put the bones in a mixed solution of methylal-acetone, seal until the bones are degreased and clean, and no yellow in the bones can be seen with the naked eye; S3. Rinse off the grease on the surface of the bones with a pressure water gun, and contain 5% to 10 Soak in water with % degreasing agent for 2-3 hours, then wash. The invention greatly shortens the degreasing time of bone specimens, the degreasing treatment of bones is clean and complete, avoiding damage to the bones, the degreasing method is simple, the degreasing effect is better and more thorough, and the degreasing time is short, which reduces the preparation of specimens to a certain extent It saves time, improves production efficiency, and has great application potential.

Description

technical field [0001] The invention belongs to the technical field of specimen preparation. Specifically, it relates to a method for defatting a bone specimen. Background technique [0002] Animal specimens play a particularly important role in biological research, teaching, and popular science work. Skeleton specimens are the main materials for studying the morphology and structure of animal bones and their relationship with other organs. Animal bone specimens not only have the function of display, but also have traditional It also plays an extremely important role in morphological taxonomy and archaeology. With the development of genetics, researchers often need to classify from both morphological and genetic aspects, which requires bone specimens of various animals for data support. Therefore, the production of animal bone specimens is particularly important. [0003] Animal bone specimens and bone specimen production techniques are also found in some textbooks and mag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00G09B23/36
CPCA01N1/00G09B23/36
Inventor 剡海阔王兴贤赖佳平肖丹媚
Owner 广州广宠生物科技有限公司
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