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A double-plasmid co-expression strain of mutant cpd photorepair enzyme and 6-4 photorepair enzyme and its application

A photorepair enzyme and co-expression technology, applied in the field of biology, can solve problems such as hindering DNA replication and transcription

Active Publication Date: 2022-06-03
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If these two pyrimidine dimers are not repaired, DNA replication and transcription are hindered

Method used

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  • A double-plasmid co-expression strain of mutant cpd photorepair enzyme and 6-4 photorepair enzyme and its application
  • A double-plasmid co-expression strain of mutant cpd photorepair enzyme and 6-4 photorepair enzyme and its application
  • A double-plasmid co-expression strain of mutant cpd photorepair enzyme and 6-4 photorepair enzyme and its application

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Embodiment 1

[0039] The log-phase Antarctic ice algae cells were extracted with the Trizol reagent of Invitrogen Company for total RNA. Tool

[0053] The target fragment was T cloned. Add the following ligation reaction solution to the microcentrifuge tube (total reaction system 10.0μ

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Abstract

The present invention provides a double-plasmid co-expression strain of CPD photorepair enzyme and 6‑4 photorepair enzyme and its application. In , the glutamic acid at position 323 of CPD photorepair enzyme was mutated to alanine. The present invention constructs a mutated CPD photorepair enzyme and 6-4 photorepair enzyme dual-plasmid co-expression strain, which simultaneously has the ability of CPD photorepair enzyme and 6-4 photorepair enzyme to effectively repair DNA damage caused by ultraviolet radiation , the strain obtained by site-directed mutation of CPD photorepair enzyme has significantly improved resistance to ultraviolet radiation, which is of great significance for the study of the impact of UV-B on organisms and the biological mechanism of organisms adapting to UV-B enhancement.

Description

A mutant CPD photorepair enzyme and 6-4 photorepair enzyme dual plasmid co-expression strain and its application technical field The invention belongs to technical field of biology, be specifically related to a kind of mutated CPD light repair enzyme and 6-4 light repair enzyme double Plasmid co-expression strains and their applications. Background technique [0002] The ozone layer can absorb more than 99% of ultraviolet rays, so that humans and other creatures on the earth will not be strongly The ozone layer is also called the "protective umbrella" of the earth due to the damage of ultraviolet radiation from the sun. However, this "protective umbrella" has been strictly severe damage, and extreme climates may also lead to more severe ozone depletion in the polar regions. The main effect of ozone depletion is to UV-B that reaches the surface is enhanced, and UV-B has adverse effects on animals and plants, and is harmful to unicellular organisms such as microalgae...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N15/70C12N15/60C12R1/19
CPCC12N9/88C12N15/70C12Y401/99003Y02A50/30
Inventor 缪锦来曲长凤安美玲郑洲
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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