Mutant CPD photo-repair enzyme and 6-4 photo-repair enzyme double-plasmid co-expression bacterial strain and application thereof
A photorepair enzyme and co-expression technology, applied in the field of biology, can solve problems such as hindering DNA replication and transcription
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[0034] The genes of CPD photorepair enzyme and 6-4 photorepair enzyme in this example come from Antarctic ice algae (Chlamydomonassp. ICE-L). The invention provides CPD photorepair enzyme (GenBank accession number: KC344405) and 6-4 photorepair enzyme (GenBank accession number: MF784511) and their expression vectors are BL21(DE3) / pET-28a(Kan+) / CPD photorepair enzyme respectively Repair enzyme and BL21(DE3) / pET-21a(Amp+) / 6-4 photorepair enzyme.
[0035] 1. Acquisition of cDNA sequences of CPD photorepair enzyme and 6-4 photorepair enzyme of Antarctic ice algae
[0036] 1.1. Cultivation of Antarctic ice algae
[0037] The Antarctic ice algae used in this example was separated and purified from seawater and sea ice samples collected near Zhongshan Station (69 ° S, 77 ° E) during China's eighteenth Antarctic scientific expedition in November 2001. It is preserved in the Antarctic ice algae species bank of the Key Laboratory of Marine Bioactive Substances of the State Oceanic Adm...
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