Method for improving yield of homoorientin produced by recombinant bacteria through coordinated regulation strategy

A technology of isoorientin and recombinant bacteria, applied in the fields of genetic engineering technology and microorganisms, can solve the problems such as the inability to greatly increase the yield, and achieve the effects of inhibiting the accumulation of acetic acid, increasing the yield and simple process
CN109402154AActive Publication Date: 2019-03-01NANJING FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING FORESTRY UNIV
Publication Date
2019-03-01

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Abstract

The invention provides a method for improving the yield of homoorientin produced by recombinant bacteria through a coordinated regulation strategy. Based on the recombinant bacteria carrying a C-glycosides glycosyl transferase Gt6CGT gene, the yield of the homoorientin produced by the recombinant bacteria is greatly improved through regulation of supply of a UDP-glucose by introducing genes, optimization of an induction conversion strategy and control of acetic acid accumulation. Through the coordinated regulation strategy, the recombinant bacteria BL21-CGT-III homoorientin yield reaches 1371mg / L, the molar conversion rate of a substrate reaches 97.4% and is 40 times the current production peak, and the process for preparing the homoorientin is simple.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering technology and microorganisms, and specifically relates to a method for improving the yield of isoorientin produced by recombinant bacteria by using a coordinated regulation strategy. Background technique

[0002] Carbon glycoside flavonoids are flavonoids whose sugar groups are directly connected to flavonoids through C-C bonds. They are widely found in wheat, corn, rice, mung beans, blueberries, lemons, black tea and bamboo leaf extracts. Compared with oxyglycoside flavones, carbon glycoside flavones have the characteristics of stable structure and ability to penetrate deep into the lesion. Isoorientin, also known as luteolin 6-C-glucoside, is a luteolin glycoside carbon glycoside flavonoid compound, and is one of the main components of flavonoids in bamboo leaf extract. Studies in recent years have found that isorientin can scavenge free radicals, resist the loss of cell viability caused by ...

Claims

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